Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7069)
Key features and details
- Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Suitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, Dot blot, WB
製品の概要
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製品名
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, Dot blot, WBmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Full length native mouse IgG (purified).
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標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads. -
特記事項(標識)
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
ポリ/モノ
ポリクローナル -
研究分野
関連製品
アプリケーション
Our Abpromise guarantee covers the use of ab7069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | 1/200 - 1/1000. | |
ELISA | 1/7000. | |
IHC-P | Use at an assay dependent dilution. | |
IHC-Fr | Use at an assay dependent dilution. | |
Immunomicroscopy | Use at an assay dependent dilution. | |
Dot blot | Use at an assay dependent dilution. | |
WB | 1/1000 - 1/2000. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7069)This image is courtesy of an anonymous Abreview.
ab13918 staining RANK in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA/5% normal goat serum/0.1% saponin for 30 minutes at 22°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 9 hours at 4°C. An alkaline phosphatase-conjugated goat anti-mouse IgG H&L (ab7069) (1/300) was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (2)
ab7069 は 2 報の論文で使用されています。
- McCarthy JJ et al. Effective fiber hypertrophy in satellite cell-depleted skeletal muscle. Development 138:3657-66 (2011). IHC-Fr . PubMed: 21828094
- Laurentino SS et al. Regucalcin is broadly expressed in male reproductive tissues and is a new androgen-target gene in mammalian testis. Reproduction 142:447-56 (2011). WB . PubMed: 21680783