Application
Immunocytochemistry/ Immunofluorescence
IF was performed on mouse cell-line. Fixation with 4% PFA for 1hr at RT, followed by blocking with 3% FCS solution. Primary antibody used from other company, diluted 1:100 in blocking solution. Incubated for 16hr, 4oC. Secondary antibody diluted 1:200 in blocking solution, incubated for 1hr at room temperature.
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投稿 Jul 29 2021