Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)

製品の概要

  • 製品名
    Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed
    IgG 二次抗体 製品一覧
  • 由来種
    Goat
  • ターゲット生物種
    Mouse
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, horse, human, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • 吸着処理血清

    Chicken, Cow, Horse, Human, Pig, Rabbit more details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 標識
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab150117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

画像

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab150117 Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • ab134375 staining CD24 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG H&L (ab150117) (1/200) was used as the secondary antibody.

    See Abreview

  • Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

    Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    Fix in 3%PFA in PBS for 30 min at RTIncubate in 7.5% sucrose-PBS for 3h at RTIncubate in 15% sucrose-PBS at 4 degree Celsius overnightEmbed the EBs in tissue-Tek OCT compoundCut frozen sections to 4-20 µm thickness

    Primary antibody 1: Mouse anti-Ki67 (ab53280), 1:50

    Primary antibody 2: Rabbit anti-laminin, 1:400
    Secondary antibody 1: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488) pre-adsorbed (ab150117), 1:200

    Secondary antibody 2: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) pre-adsorbed (ab150084), 1:300
    Nuclei were counterstained with DAPI

     

参考文献

This product has been referenced in:
  • Hu Y  et al. Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function. Theranostics 8:169-184 (2018). Read more (PubMed: 29290800) »
  • Furusho A  et al. Involvement of B Cells, Immunoglobulins, and Syk in the Pathogenesis of Abdominal Aortic Aneurysm. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29545260) »

See all 40 Publications for this product

レビューと Q&A

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Application
Immunocytochemistry/ Immunofluorescence
Following 4% PFA fixation, cells from the ventral mesencephalon were incubated with an anti-GFAP (Non-Abcam) antibody O/N @ RT. ab150117 (1:500) was applied for 2 hours @ RT to visualise astrocytes.
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Abcam user community

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投稿 Jun 04 2018

very good

Excellent
Abreviews
Application
Immunohistochemistry (PFA fixed)
We use this antibody together with the antibody 19905 (EPX) in eosinophilic skin.
As working concentration we used a 1/100 Dilution for 30 minutes at room temperature.
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投稿 Feb 14 2018

Application
Immunohistochemistry (Frozen sections)
Adult rabbit testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B ) anti-DDX4 (Abcam ab27591) for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Goat-Anti Rabbit 488 (Abcam ab150117) applied at a 1:500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.
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Mr. Bryan Niedenberger

Verified customer

投稿 Mar 29 2016

Application
Immunocytochemistry/ Immunofluorescence
This antibody has been used extensively with a number of different primary antibodies generated in rabbits. It performs extremely well at a 1:200 dilution.
Attached is an immunofluorescent image of paraformaldehyde-fixed HeLa cells that were immunofluorescently labeled with a cell membrane-specific antibody (ab134375). The secondary antibody has been pseudo-colored green in the merged image. DNA was counterstained with DAPI.
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Dr. Kirk Mcmanus

Verified customer

投稿 Mar 28 2014

Application
Immunocytochemistry/ Immunofluorescence
Following fixation in 4% PFA, the cells were assessed for alpha smooth muscle Actin using 1:100 dilution of the primary antibody (ab7817) in 1% serum, 0.1% triton, 0.1% BSA in PBS, followed by detection using goat polyclonal mouse IgG Alexa 488 (ab150117) at 1:500. The results show clear alpha smooth muscle Actin fibres (green). An isotype control IgG was run in parallel and showed no positive staining (not shown here). Nuclei were counterstained using DAPI (blue).
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投稿 Sep 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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