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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

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  • SDS
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • IHC - Wholemount - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

Key features and details

  • Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-P

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
ブロッキング
Product image
Normal Goat Serum (ab7481)

関連製品

製品の概要

  • 製品名

    Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)
    IgG 二次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488)
  • 由来種

    Goat
  • ターゲット生物種

    Mouse
  • 特異性

    Specific to mouse IgG.

  • アプリケーション

    適用あり: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-Pmore details
  • 免疫原

    Other Immunogen Type corresponding to Mouse IgG.

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究分野

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • Alexa Fluor® 488

関連製品

  • Alternative Versions

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    • Goat Anti-Mouse IgG H&L (ab182017)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (Biotin) (ab207996)
  • Related Products

    • Normal Goat Serum (ab7481)

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr (3)
Use at an assay dependent concentration.
ICC/IF (5)
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/2000.

ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

IHC-P (3)
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/2000.

ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

IHC-P
Use at an assay dependent concentration.

画像

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

    ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab150113 Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

    Overlay histogram showing HeLa cells stained with ab29 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29, 0.1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

    Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.

    These data were developed using the unconjugated antibody (ab182017).

  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

    Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    These data were developed using the unconjugated antibody (ab182017).

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

    Overlay histogram showing SV40LT-SMC cells stained with ab7817 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab7817, 0.1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 dilution for 30 min at 22°C
    Isotype control antibody (black line) was mouse IgG2a [18C8BC7AD10] (ab170191) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

    Overlay histogram showing HeLa cells stained with ab26 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab26, 1µg/1x10^6 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • IHC - Wholemount - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    IHC - Wholemount - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)This image is courtesy of an anonymous Abreview.

    IHC - Wholemount of Caenorhabditis elegans embryo labelling RNA polymerase II CTD repeat YSPTSPS with ab817. Sample was incubated with primary antibody (1/100 in PBS + 3% BSA + 0.1% Triton-X 100) for 24 hours at 4°C. ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.

    See Abreview

  • Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (259)

    ab150113 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab150113 は 259 報の論文で使用されています。

    • Zhang Y  et al. Knockout of beta-2 microglobulin reduces stem cell-induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR-24/Bim pathway. J Cell Mol Med 24:695-710 (2020). PubMed: 31729180
    • Wang J  et al. Spermidine alleviates cardiac aging by improving mitochondrial biogenesis and function. Aging (Albany NY) 12:650-671 (2020). PubMed: 31907336
    • Tang C  et al. Auxiliary genetic analysis in a Chinese adolescent NPH family by single nucleotide polymorphism screening. Mol Med Rep 21:1115-1124 (2020). PubMed: 31922211
    • Barbeito-Andrés J  et al. Congenital Zika syndrome is associated with maternal protein malnutrition. Sci Adv 6:eaaw6284 (2020). PubMed: 31950075
    • Wu A  et al. Transmissible gastroenteritis virus targets Paneth cells to inhibit the self-renewal and differentiation of Lgr5 intestinal stem cells via Notch signaling. Cell Death Dis 11:40 (2020). PubMed: 31959773
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 13 Abreviews or Q&A

    Immunofluorescence

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    HeLa cells were imaged using ab150113 as a secondary antibody.

    Fix cells for 5 min with 4% paraformaldehyde, permeabilize for 5 min with 0.1% Triton-X 100

    Primary antibody: ab17355, MYC, 1:100 dilution, RT, 90 min

    Secondary antibody: ab150113, 1:500 dilution, RT, 45 min

    Abcam user community

    Verified customer

    投稿 Feb 05 2021

    Excellent staining of CD45+ cells (human spleen)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    This secondary antibody works great by IF imaging. Even though it is a direct conjugate.
    Concentration: 1:500 for 30 min at RT
    primary AB: CD45
    tissue: human spleen

    Abcam user community

    Verified customer

    投稿 Dec 01 2020

    Excellent antibody for IF on frozen tissue sections

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Very specific and intense staining of cytokeratin14 in skin tissue sections.
    Inc time of secondary antibody: 30 min
    Concentation: 1:500
    Temperature: room temparature

    Abcam user community

    Verified customer

    投稿 Nov 16 2020

    Perfect working secondary antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Secondary antibody gave very specific signals without any background.

    Cells: Melanoma cell
    Fixation: Acetone
    Primary antibody: Y14 ab5828
    Secondary antibody: ab150113 with a concentration of 1:500, 30 min, RT

    Abcam user community

    Verified customer

    投稿 Oct 03 2020

    primary: mouse anti β-Amyloid Clone 6E10 (BioLegend) secondary: ab150113

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    1:500 dilution in Blocking Solution (10% NGS, 0,3% TritonX100)

    Incubation time: 1h

    Good staining results!

    Abcam user community

    Verified customer

    投稿 Jun 22 2020

    Anti-mouse secondary successful IHC-P

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    It worked great, 1/200 concentration.

    Abcam user community

    Verified customer

    投稿 May 26 2020

    Immunocytochemical staining (HeLa cells)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Cell: HeLa
    Fix & permeabilization: 4% formalin, 0.03% Triton-X100
    Blocking: 3% BSA/PBS
    1st antibody: anti-Nup133 mouse monoclonal antibody (1/500 dil, 3% BSA/PBS)
    2nd antibody: Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) (1/2500 dil, 3% BSA/PBS)

    Abcam user community

    Verified customer

    投稿 Feb 24 2020

    a-tubulin staining on RK-13 (rabbit kidney cell line)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Application: Immunocytochemistry/ Immunofluorescence
    Cells were fixed in 10% neutral buffered formalin.
    Permeabilization: Triton X-100
    Primary antibody: Mouse monoclonal anti-a-tubulin
    Blocking: 5% BSA in PBS
    Counterstained with DAPI

    Dr. Egi Kardia

    Verified customer

    投稿 Apr 29 2019

    Calnexin staining on RK-13 (rabbit kidney cell line)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Resulted in bright and vibrant staining.
    Cells were fixed in 10% neutral buffered formalin.
    Permeabilization: Triton X-100
    Primary antibody: Rabbit polyclonal anti-calnexin (ab75801)
    Blocking: 5% BSA in PBS
    Counterstained with DAPI

    Abcam user community

    Verified customer

    投稿 Dec 07 2018

    Whole-mount immunofluorescence on mouse inner ear sensory epithelia

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    I used this product by Abcam to stain the sensory epithelia of mouse inner ears.
    Fixed samples were incubated overnight (4 c) with primary mouse anti myo7a antibody and on the next morning washed 3 times in PBS and then incubated for 2 hours in Abcam's goat anti mouse 488 antibody (ab150113), as well as DAPI and 647 phalloidin for 2 hours in room temp.
    After mounting samples were imaged with Leica SP8 confocal microscope.
    Staining shows specificity for hair cells.

    Mr. Shahar Taiber

    Verified customer

    投稿 Oct 31 2018

    1-10 of 13 Abreviews or Q&A

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