Goat Anti-Human IgG H&L (HRP) preadsorbed (ab7153)

Submit a review Q&A (3)References (3)

Key features and details

  • Goat Anti-Human IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, WB

製品の概要

  • 製品名

    Goat Anti-Human IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Human

  • 特異性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.

  • アプリケーション

    適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native human IgG (purified).

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Horseradish Peroxidase (HRP)

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7153の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/500 - 1/2500.
Dot blot
Use at an assay dependent dilution.
ELISA
1/7000 - 1/200000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
特記事項
ICC/IF
1/500 - 1/2500.
Dot blot
Use at an assay dependent dilution.
ELISA
1/7000 - 1/200000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab7153 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7153 は 3 報の論文で使用されています。

  • Bell BN  et al. Neutralizing antibodies targeting the SARS-CoV-2 receptor binding domain isolated from a naïve human antibody library. Protein Sci 30:716-727 (2021). PubMed: 33586288
  • Uppu DSSM  et al. Temporal release of a three-component protein subunit vaccine from polymer multilayers. J Control Release 317:130-141 (2020). PubMed: 31756392
  • Henson D  et al. Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report. Arterioscler Thromb Vasc Biol 39:1884-1892 (2019). PubMed: 31315438

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

Dear Tech Support Team,

Kindly advise if the following options of the secondary ab will work in Elisa settings to quantify interaction with the attached primary mAb (it is IgG3 λ chain ):

https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-HRP-pre-adsorbed-ab102429.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Human-IgG-HL-HRP-pre-adsorbed-ab7153.html

Thanks in advance for your assistance.

Have a nice week,

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Answer

Thank you for contacting us and your interest in our products.
Both of the secondary antibodies chosen (ab102429 and ab7153) should be able to detect the primary antibody (human IgG3) in the ELISA the customer is hoping to perform.
I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Question

Is there any difference between Human IgG antibodies ab6858 and ab7153?

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Answer

Thank you for your recent telephone enquiry. I can confirm that these two antibodies are essentially the same. The difference is that ab7153 is further purified by preadsorbtion to minimize its cross reactivity to 10 serum other serum proteins. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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Question

Does this antibody recognize all IgG subtypes?

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Answer

Thank you for your enquiry. These antibodies should recognize all human IgG subclasses. Please contact us again if you require further information.

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