Goat Anti-Human IgG H&L (HRP) (ab6858)

Submit a review Q&A (4)References (16)
Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Key features and details

    • Goat Anti-Human IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

    製品の概要

    • 製品名

      Goat Anti-Human IgG H&L (HRP)
      IgG 二次抗体 製品一覧

    • 由来種

      Goat

    • ターゲット生物種

      Human

    • アプリケーション

      適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

    • 免疫原

      human IgG whole molecule

    • 標識

      HRP

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 精製度

      Affinity purified

    • 特記事項（精製）

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 特記事項（標識）

      Horseradish Peroxidase (HRP)

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    関連製品

      アプリケーション

      Our Abpromise guarantee covers the use of ab6858 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      アプリケーション Abreviews 特記事項
      ICC/IF 1/1000 - 1/5000.
      Dot blot Use at an assay dependent concentration.
      ELISA Use at an assay dependent dilution.
      IHC-P Use at an assay dependent concentration.
      IHC-Fr Use at an assay dependent concentration.
      Immunomicroscopy Use at an assay dependent concentration.
      WB 1/5000 - 1/25000.

      画像

      • Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
        Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

        Dot blot analysis of human IgG using ab6858 at 1/1000 dilution.

        The indicated qualtities of human IgG were spotted. Blocking was performed for 1 hour at room temperature using a proprietary blocking buffer prior to addition of ab6858.

         

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (16)

      ab6858 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6858 は 16 報の論文で使用されています。

      • Yang Y  et al. Reduction in milk fat globule-EGF factor 8 inhibits triple-negative breast cancer cell viability and migration. Oncol Lett 17:3457-3465 (2019). PubMed: 30867784
      • Niu X  et al. Convalescent patient-derived monoclonal antibodies targeting different epitopes of E protein confer protection against Zika virus in a neonatal mouse model. Emerg Microbes Infect 8:749-759 (2019). PubMed: 31130109
      • Nika L  et al. An HER2-Displaying Virus-Like Particle Vaccine Protects from Challenge with Mammary Carcinoma Cells in a Mouse Model. Vaccines (Basel) 7:N/A (2019). PubMed: 31137559
      • Li ZY  et al. MicroRNA-4500 suppresses tumor progression in non-small cell lung cancer by regulating STAT3. Mol Med Rep 20:4973-4983 (2019). PubMed: 31638206
      • D'Gama JD  et al. A Conserved Streptococcal Virulence Regulator Controls the Expression of a Distinct Class of M-Like Proteins. mBio 10:N/A (2019). PubMed: 31641092
      View all Publications for this product

      レビューと Q&A

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      1-4 of 4 Abreviews or Q&A

      Question

      Hi, I would like to know whether this Ab cross reacts with IgGs from any other species. If yes, are there any Abs from your company that recognizes specifically human IgGs? Thank you

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      Answer

      Anti-Human IgG antibody ab6858 has not been tested for reactivity with other species. For an antibody that is exclusively reactive with human IgG, we recommend ab97175.

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      Question

                      Hello,   I purchased the Goat polyclonal Secondary Antibody to Human IgG - H&L (HRP) (ab6858). May I ask about the dilution factor for this antibody in ELISA? Thank you very much!   Best regards,  

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      Answer

      Thank you for contacting us. For this secondary antibody we recommend a 1/180K dilution with ELISA. However, you will need to optimise and determine the best dilutions for your specific assay; considering starting dilutions from 1/40000. I hope this information is helpful to you.

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      Question

      I want to purchase goat anti-human IgG secondary antibody conjugated with HRP for ELISA. I want to test the autoantibodies in human serum. What is the difference between pre-adsorbed and none pre-adsorbed antibody? Which one is better for my test? Thank you very much!  Best regards,  

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      Answer

      Thank you for contacting us. Pre-adsorbed antibodies are more specific towards primary antibodies than the non pre-adsorbed products. Usually, a conjugated antibody is purified by affinity chromatography using an antigen of interest coupled to agarose beads. The conjugated antibody binds its antigen on the column and will then be eluted. Pre-adsorption purifications can be performed to further increase the specificity of a secondary antibody. Adsorption chromatography involves a stationary phase as an adsorbent. Conjugated antibodies are then passed through gel containing immobilized serum proteins from potentially cross-reactive species. The resulting purified antibody is more pure and specific significantly reducing cross reactivity. Pre-adsorbed secondary antibodies reduce non-specific background staining, especially in IHC when working with closely homologous species I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      Is there any difference between Human IgG antibodies ab6858 and ab7153?

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      Answer

      Thank you for your recent telephone enquiry. I can confirm that these two antibodies are essentially the same. The difference is that ab7153 is further purified by preadsorbtion to minimize its cross reactivity to 10 serum other serum proteins. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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