Key features and details
- Goat Anti-Human IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: Flow Cyt
保存方法Shipped at 4°C. Store at +4°C.
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Specific antibodies were absorpted by incubation with Sepharose-bound human immuno- globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human immunoglobulins a minimum of 65% protein bound.
特記事項（標識）The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
特記事項F/P ratio is 4-6 mol Fluorescein per 1 mol of goat Ig. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where Ig's are depostited.
Our Abpromise guarantee covers the use of ab8503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.