Key features and details
- Goat Anti-Human IgA H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: IHC-P
製品名Goat Anti-Human IgA H&L (FITC)
IgA 二次抗体 製品一覧
特異性Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumin fractions of animal species.
アプリケーション適用あり: IHC-Pmore details
標識FITC. Ex: 493nm, Em: 528nm
保存方法Shipped at 4°C. Store at +4°C.
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
特記事項（標識）The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgG.
Our Abpromise guarantee covers the use of ab8500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.