Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed (ab6275)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy
製品の概要
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製品名
Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins. -
アプリケーション
適用あり: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details -
吸着処理血清
Cow, Horse, Human more details -
免疫原
Rat IgG F(ab')2 fragment
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate
BSA is IgG and protease free -
Concentration information loading...
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特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
Our Abpromise guarantee covers the use of ab6275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | 1/500 - 1/5000. | |
Dot blot | Use at an assay dependent dilution. | |
WB | 1/2000 - 1/10000. | |
ELISA | 1/20000 - 1/400000. | |
IHC | Use at an assay dependent dilution. | |
Immunomicroscopy | Use at an assay dependent dilution. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (2)
ab6275 は 2 報の論文で使用されています。
- Wu X et al. Hydrogen exerts neuroprotective effects on OGD/R damaged neurons in rat hippocampal by protecting mitochondrial function via regulating mitophagy mediated by PINK1/Parkin signaling pathway. Brain Res 1698:89-98 (2018). PubMed: 29958907
- Mochida GH et al. CHMP1A encodes an essential regulator of BMI1-INK4A in cerebellar development. Nat Genet 44:1260-4 (2012). PubMed: 23023333