Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed (ab6275)

Submit a review Q&A (1)References (2)

Key features and details

  • Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy

製品の概要

  • 製品名

    Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Rat

  • 特異性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.

  • アプリケーション

    適用あり: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • 吸着処理血清


    Cow, Horse, Human more details

  • 免疫原

    Rat IgG F(ab')2 fragment

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 特記事項（標識）

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

関連製品

    アプリケーション

    Our Abpromise guarantee covers the use of ab6275 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    ICC/IF 1/500 - 1/5000.
    Dot blot Use at an assay dependent dilution.
    WB 1/2000 - 1/10000.
    ELISA 1/20000 - 1/400000.
    IHC Use at an assay dependent dilution.
    Immunomicroscopy Use at an assay dependent dilution.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab6275 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6275 は 2 報の論文で使用されています。

    • Wu X  et al. Hydrogen exerts neuroprotective effects on OGD/R damaged neurons in rat hippocampal by protecting mitochondrial function via regulating mitophagy mediated by PINK1/Parkin signaling pathway. Brain Res 1698:89-98 (2018). PubMed: 29958907
    • Mochida GH  et al. CHMP1A encodes an essential regulator of BMI1-INK4A in cerebellar development. Nat Genet 44:1260-4 (2012). PubMed: 23023333

    レビューと Q&A

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    Question

    I need a anti-rat biotin conjugated secondary Ab raised in goat to perform IHC on human joint tissue. In addition to ab6275, you have ab6619, 6256, 7176 in your catalog that match that description. Can you tell me if there is any difference between these antibodies and which one you recommend for this application (IHC)?

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    Answer

    Thank you for your enquiry. Ab6275: Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(ab')2 [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(ab')2. Ab7176: Biotin Conjugated Fab Fragment of Affinity Purified anti-Rat IgG [H&L] [Goat] - This product is the Fab portion (single Fab fragments) of the Goat IgG raised against Rat IgG. Ab6256:Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(c) [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(c). Typically Fab and Fab2 fragments will bind less than the entire IgG molecule. This means these types of products are good for assays in which very low background is desired. I hope I was able to make things more clear. Please do not hesitate to contact me if you have any other questions regarding this issue.

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