Key features and details
- Goat F(ab')2 Anti-Rabbit IgG - H&L (TRITC ), pre-adsorbed
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, Flow Cyt, Immunomicroscopy
製品名Goat F(ab')2 Anti-Rabbit IgG - H&L (TRITC ), pre-adsorbed
IgG 二次抗体 製品一覧
特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
アプリケーション適用あり: ICC/IF, ELISA, Flow Cyt, Immunomicroscopymore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rabbit IgG whole molecule
標識TRITC. Ex: 547nm, Em: 572nm
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
特記事項（精製）This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
特記事項（標識）Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.3 moles TRITC per mole of Goat IgG F(ab’)2
Our Abpromise guarantee covers the use of ab6011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|ELISA||1/10000 - 1/50000.|
|Flow Cyt||1/500 - 1/2500.|
|Immunomicroscopy||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.