Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed (ab6013)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
製品の概要
-
製品名
Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
IgG 二次抗体 製品一覧 -
製品の詳細
Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins. -
アプリケーション
適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Rabbit IgG, whole molecule
-
標識
HRP
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
-
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
Our Abpromise guarantee covers the use of ab6013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | 1/500 - 1/2500. | |
Dot blot | Use at an assay dependent dilution. | |
WB | 1/2000 - 1/10000. | |
ELISA | 1/115000. | |
Electron Microscopy | Use at an assay dependent dilution. | |
IHC | Use at an assay dependent dilution. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (4)
ab6013 は 4 報の論文で使用されています。
- Ibrahim B et al. Albumin promotes proliferation of G1 arrested serum starved hepatocellular carcinoma cells. PeerJ 8:e8568 (2020). PubMed: 32185103
- Westcott NP et al. Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress. Nat Chem Biol 13:302-308 (2017). PubMed: 28092360
- Chou MH et al. Effects of hepatocyte CD14 upregulation during cholestasis on endotoxin sensitivity. PLoS One 7:e34903 (2012). WB . PubMed: 22511970
- Fuhs SR & Insel PA Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization. J Biol Chem 286:14830-41 (2011). PubMed: 21362625