Key features and details
- Goat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
製品名Goat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase)
IgG 二次抗体 製品一覧
特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(ab')2 or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
アプリケーション適用あり: ICC/IF, Dot blot, WB, ELISA, IHCmore details
Rabbit IgG F(ab')2 fragment
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
特記事項（精製）This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
特記事項（標識）Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
Our Abpromise guarantee covers the use of ab6024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/600 - 1/2800.
IHC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/600 to 1/2800 of the reconstitution concentration is suggested for this product.
Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.