Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHC
製品の概要
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製品名
Goat F(ab')2 Anti-Mouse IgM mu chain (HRP)
IgM 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. -
アプリケーション
適用あり: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
免疫原
Mouse IgM mu chain
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.87% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/500 - 1/5000.
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|
IHC-Fr |
Use at an assay dependent dilution.
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|
Dot blot |
1/5000 - 1/100000.
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|
WB |
1/2000 - 1/10000.
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|
ELISA |
1/90000.
|
|
Electron Microscopy |
Use at an assay dependent dilution.
|
|
IHC |
1/500 - 1/5000.
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特記事項 |
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ICC/IF
1/500 - 1/5000. |
IHC-Fr
Use at an assay dependent dilution. |
Dot blot
1/5000 - 1/100000. |
WB
1/2000 - 1/10000. |
ELISA
1/90000. |
Electron Microscopy
Use at an assay dependent dilution. |
IHC
1/500 - 1/5000. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (12)
ab5930 は 12 報の論文で使用されています。
- Nagase H et al. Reliable and sensitive detection of glycosaminoglycan chains with immunoblots. Glycobiology 31:116-125 (2021). PubMed: 32614944
- Casagrande FVM et al. The imbalance between dynamic and stable microtubules underlies neurodegeneration induced by 2,5-hexanedione. Biochim Biophys Acta Mol Basis Dis 1866:165581 (2020). PubMed: 31672549
- Hou X et al. Wnt signaling regulates cytosolic translocation of connexin 43. Am J Physiol Regul Integr Comp Physiol 317:R248-R261 (2019). PubMed: 31067079
- Lynn MA et al. Early-Life Antibiotic-Driven Dysbiosis Leads to Dysregulated Vaccine Immune Responses in Mice. Cell Host Microbe 23:653-660.e5 (2018). PubMed: 29746836
- Xu W et al. Emission of sulfur dioxide from polyurethane foam and respiratory health effects. Environ Pollut 242:90-97 (2018). PubMed: 29960929