Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)

Submit a review Q&A (3)References (2)

Key features and details

  • Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Flow Cyt, ELISA

製品の概要

  • 製品名

    Goat F(ab')2 Anti-Mouse IgM mu chain (FITC)
    IgM 二次抗体 製品一覧

  • 製品の詳細

    Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (FITC)

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • 特異性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt, ELISAmore details

  • 免疫原

    Mouse IgM mu chain

  • 標識

    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
  • Concentration information loading...

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 特記事項（標識）

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Goat IgG F(ab’)2

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

画像

  • Flow Cytometry - Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)
    Flow Cytometry - Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)
    Ab5926 was used at dilution 1/100 with the primary antibody ab2784 in Flow Cyt. See the review on ab2784.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab5926 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5926 は 2 報の論文で使用されています。

  • Zhong Y  et al. Spheres derived from the human SK-RC-42 renal cell carcinoma cell line are enriched in cancer stem cells. Cancer Lett 299:150-60 (2010). Flow Cyt . PubMed: 20846785
  • Wang K  et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009). ICC/IF ; Rhesus monkey, Human . PubMed: 20025521

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

human blood cells confirmed by PCR to be B35 positive. secondary rat IGM supposed to react with mouse. primary 1/10 to neat. FACS

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Answer

Hello and thank you for your patience is trying to troublshooting your Flow Cytometry experiment. I have noticed the secondary you are using is specific to the mu chain of mouse IgM. The primary antibodies are to total IgM, so this is likely the reason you are only getting a portion of the staining. I would suggest using a secondary like ab47830.

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Question

Dear Sir/Madam,

I am writing for technical support. Recently, we have purchased a vial of mouse anti-connexin43 antibody (ab11369) from Abcam. We didn't notice that it is IgM isotype, thus we did not get positive signals from our first trial of this antibody. We are now considering purchase a vial of goat anti-mouse IgM antibody (ab5926). We are wondering whether this one reacts with other normally used secondary antibodies, such as goat anti-rabbit IgG and goat anti-mouse IgG, as we are going to do some co-stainings.

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Answer

Thank you for contacting us.

ab5926 shows less than 1% cross reactivity against other mouse heavy or light chain isotypes in ELISA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Does this cross-react with human IgM ? I am looking for a Fab-fragment secondary antibody to human IgM for use in flow cytometry and possibly also confocal microscopy.

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Answer

Thank you for your enquiry. Ab5926 has not been tested for cross-reactivity with human IgM, just mouse IgM. We do have several human IgM secondary antibodies (for example ab8506), but unfortunately not a Fab-fragment secondary antibody to human IgM. To see if another company may have what you are looking for, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogues of all online antibody companies (currently 249). If you have any additional questions, please contact us again.

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