Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)
Key features and details
- Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Flow Cyt, ELISA
製品の概要
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製品名
Goat F(ab')2 Anti-Mouse IgM mu chain (FITC)
IgM 二次抗体 製品一覧 -
製品の詳細
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (FITC) -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. -
アプリケーション
適用あり: ICC/IF, Flow Cyt, ELISAmore details -
免疫原
Mouse IgM mu chain
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標識
FITC. Ex: 493nm, Em: 528nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA -
Concentration information loading...
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特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Goat IgG F(ab’)2 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
Our Abpromise guarantee covers the use of ab5926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | 1/1000 - 1/5000. | |
Flow Cyt | 1/500 - 1/2500. | |
ELISA | 1/10000 - 1/50000. |
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (2)
ab5926 は 2 報の論文で使用されています。
- Zhong Y et al. Spheres derived from the human SK-RC-42 renal cell carcinoma cell line are enriched in cancer stem cells. Cancer Lett 299:150-60 (2010). Flow Cyt . PubMed: 20846785
- Wang K et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009). ICC/IF ; Rhesus monkey, Human . PubMed: 20025521