Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cyt
製品の概要
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製品名
Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
IgM 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). -
アプリケーション
適用あり: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details -
免疫原
Mouse IgM mu heavy chain
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA -
Concentration information loading...
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特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5929の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500 - 1/2500.
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|
ELISA |
1/10000 - 1/50000.
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|
WB |
1/1000 - 1/5000.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
IHC |
1/500 - 1/5000.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
Flow Cyt |
1/200 - 1/1000.
|
特記事項 |
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ICC/IF
1/500 - 1/2500. |
ELISA
1/10000 - 1/50000. |
WB
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
IHC
1/500 - 1/5000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
1/200 - 1/1000. |
画像
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ab5929 was used as the secondary antibody in WB.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab5929 は 3 報の論文で使用されています。
- Liu HY et al. Leveraging the Treg-intrinsic CTLA4-PKC? signaling pathway for cancer immunotherapy. J Immunother Cancer 9:N/A (2021). PubMed: 34588224
- Hong R et al. Distinct roles of BCNP1 in B-cell development and activation. Int Immunol 32:17-26 (2020). PubMed: 31412363
- Grewal N et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . PubMed: 25502628