Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)

Submit a review Q&A (3)References (6)

Key features and details

  • Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC

製品の概要

  • 製品名

    Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed
    IgG + IgM + IgA 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • 特異性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c). This reagent is suitable for the detection of all mouse subclasses, isotypes and chain combinations.

  • アプリケーション

    適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details

  • 吸着処理血清


    Human more details

  • 免疫原

    Mouse IgG, IgA and IgM whole molecules

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

  • 特記事項（標識）

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/120000. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

画像

  • Western blot - Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)
    Western blot - Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)
    Ab6006 was used at dilution 1/3000 with the primary antibody ab2861 in WB. See the review on ab2861.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (6)

ab6006 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6006 は 6 報の論文で使用されています。

  • He FL  et al. Covering the proximal nerve stump with chondroitin sulfate proteoglycans prevents traumatic painful neuroma formation by blocking axon regeneration after neurotomy in Sprague Dawley rats. J Neurosurg N/A:1-11 (2020). PubMed: 32470939
  • Bassagañas S  et al. Pancreatic cancer cell glycosylation regulates cell adhesion and invasion through the modulation of a2ß1 integrin and E-cadherin function. PLoS One 9:e98595 (2014). WB . PubMed: 24878505
  • Wang Y  et al. Comparison between the efficacies of curcumin and puerarin in C57BL/6 mice with steatohepatitis induced by a methionine- and choline-deficient diet. Exp Ther Med 7:663-668 (2014). WB . PubMed: 24520264
  • Patel P  et al. The endogenous production of hydrogen sulphide in intrauterine tissues. Reprod Biol Endocrinol 7:10 (2009). WB . PubMed: 19200371
  • Adinolfi E  et al. Expression of the P2X7 receptor increases the Ca2+ content of the endoplasmic reticulum, activates NFATc1, and protects from apoptosis. J Biol Chem 284:10120-8 (2009). WB . PubMed: 19204004
  • Chen WY  et al. Cl- Is Required for HCO3- Entry Necessary for Sperm Capacitation in Guinea Pig: Involvement of a Cl-/HCO3- Exchanger (SLC26A3) and CFTR. Biol Reprod : (2008). PubMed: 18784352

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

Can you please check if ab6006 is suitable for all the three below primary antibodies ? Application is WB.

1) Ab119484

2) Ab80039

3) Ab110304

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Answer

Thank you for contacting us.

I can confirm that ab6006 is suitable for use with antibodies ab119484, ab80039 and ab110304.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Customer want to use ab6006 as secondary antibody with ab110304 & ab80039 for

Chemiluminiscence.



Is it ok ?



Regards

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Answer

Thank you for your inquiry.

I am happy to confirm that ab6006 is a suitable secondary antibody for these two primary antibodies:

ab80039 and ab110304.

ab6006 detects the heavy and light chains of mouse IgG and IgM and IgA. ab80039 and ab110304 are both of the mouse IgG isotype.

I hope this information is helpful. Please do not hesitate to contact me again with any further questions your customer might have.

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Question

Customer Question: how many peroxidases iare there on the antibody fragment? is it on the antigen binding site or on other sites? does it interfere with antigen binding?

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Answer

Thank you for your enquiry. The originator of this antibody conjugated at a 2:1 ratio. There could be 1 or 2 peroxidase per antibody molecule. Peroxidase molecule randomly bind to Lysines of the antibody. If there are lysines at the antigen binding site, the enzyme could block it sometimes. That is why the titer of an antibody usually drops a little bit after conjugation. But it doesn't meant that the antibody is being affected. If you have further questions, please contact us again and we will gladly assist you.

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