Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
製品名Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed
IgG + IgM + IgA 二次抗体 製品一覧
特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c). This reagent is suitable for the detection of all mouse subclasses, isotypes and chain combinations.
アプリケーション適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Mouse IgG, IgA and IgM whole molecules
保存方法Shipped at 4°C. Store at +4°C.
バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
特記事項（精製）This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
特記事項（標識）Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab6006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/2500.|
|Dot blot||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
|ELISA||1/120000. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.|
|Electron Microscopy||Use at an assay dependent dilution.|
|IHC||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6006 は 6 報の論文で使用されています。
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