Goat F(ab')2 Anti-Mouse IgG H&L (PE) preadsorbed (ab7002)

Submit a review Q&A (8)References (5)

Key features and details

  • Goat F(ab')2 Anti-Mouse IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr

製品の概要

  • 製品名

    Goat F(ab')2 Anti-Mouse IgG H&L (PE) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details

  • 吸着処理血清


    Cow, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native Mouse IgG (purified).

  • 標識

    PE. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

  • 特記事項（標識）

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 特記事項


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7002の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/250.
Immunomicroscopy
Use at an assay dependent dilution.
Flow Cyt
1/100 - 1/250. The maximum amount of Ab need to stain 106 cells in flow cytometry is 1.0 ug.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
特記事項
ICC/IF
1/100 - 1/250.
Immunomicroscopy
Use at an assay dependent dilution.
Flow Cyt
1/100 - 1/250. The maximum amount of Ab need to stain 106 cells in flow cytometry is 1.0 ug.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (5)

ab7002 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7002 は 5 報の論文で使用されています。

  • Hill PJ  et al. Modifications of Pseudomonas aeruginosa cell envelope in the cystic fibrosis airway alters interactions with immune cells. Sci Rep 7:4761 (2017). Flow Cyt . PubMed: 28684799
  • Ju L  et al. Von Willebrand factor-A1 domain binds platelet glycoprotein Iba in multiple states with distinctive force-dependent dissociation kinetics. Thromb Res 136:606-12 (2015). PubMed: 26213126
  • Bârsan L  et al. Bone marrow iron distribution, hepcidin, and ferroportin expression in renal anemia. Hematology 20:543-52 (2015). PubMed: 25745821
  • Cai D  et al. Improved tools for the Brainbow toolbox. Nat Methods 10:540-7 (2013). PubMed: 23817127
  • Schulz U  et al. Interleukin-10 Promotes NK Cell Killing of Autologous Macrophages by Stimulating Expression of NKG2D Ligands. Scand J Immunol 72:319-31 (2010). Flow Cyt . PubMed: 20883317

レビューと Q&A

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1-8 of 8 Abreviews or Q&A

Question

thank you very much for this kind offer. As we plan to use the TLR4 antibody with rat and human cells we would like to use the ab91532 product as isotype control.

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Answer

I have issued a free of charge replacement for one vial of ab91532. To check the status of the order please contact our Customer Service team and reference this number. Please be aware this product is currently out of stock. Expect delivery around December 21. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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Question

we are using your product nr ab7002 as isotype control for your TLR-4-antibody (nr.ab45104). As we want to identify TLR4 on monocytes we use an ED9-antibody, too. We know have a problem with the isotype control, which seems to bind to the ED-9-antibody, as you can see in the attached files. We now were wondering how this could happen, as the isotype control should not bind my ED-9-antibody. Furthermore we would like to know, what you suggest as to how we should proceed?

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Answer

Thank you for contacting us. The product ab7002 is not an isotype control, but a secondary antibody that binds to antibodies raised in mouse (such as ab45104). I am not certain which ED9-antibody (ab34086 Anti-SIRP alpha antibody [ED9] ??) you are refering to, but am not surprised that you see cross-reactivity as ab7002 is a secondary antibody and is supposed to cross react with mouse monoclonal antibodies. A true isotype control for the TLR4 antibody (mouse monoclonal IgG2b, PE-conjugated) would be for instance: ab91532 - Mouse IgG2b [PLPV219] (Phycoerythrin) - Isotype Control https://www.abcam.com/Mouse-IgG2b-PLPV219-Phycoerythrin-Isotype-Control-ab91532.html ab1284 - Mouse IgG2b [HybIgG2b] (Phycoerythrin) https://www.abcam.com/Mouse-IgG2b-HybIgG2b-Phycoerythrin-ab1284.html ab118540 - Mouse IgG2b monoclonal PE - Isotype control https://www.abcam.com/Mouse-IgG2b-monoclonal-PE-Isotype-control-ab118540.html And a true isotype control for the ED-antibody ab34086 - if this is the antibody you are refering to - (mouse monoclonal IgG1, unconjugated) would be for instance: ab91353 - Mouse IgG1 [ICIGG1] - Isotype Control https://www.abcam.com/Mouse-IgG1-ICIGG1-Isotype-Control-ab91353.html ab81032 - Mouse IgG1 Monoclonal (NCG01) - Isotype Control - BSA and Azide Free https://www.abcam.com/Mouse-IgG1-Monoclonal-NCG01-Isotype-Control-BSA-and-Azide-Free-ab81032.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

  I would like to ask, in order to be absolutely certain, whether your secondary antibodies, mentioned below, contain Fc part: Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7002) Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7003)   I would like to use them in the experiment where their binding to the Fc receptors would be an important issue.  

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Answer

Thank you for contacting us.  Our F(ab')2 fragment secondary antibodies ab7002 and ab7003 will not contain the Fc region, and this will be covered under our Abpromise guarantee.  If any Fc reactivity is observed in your experiments, we would be happy to offer you a free of charge replacement, credit, or refund.  I hope this helps, please let me know if you need any additional information. 

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Question

Thank you for the informations. Is it possible to know approximately when you will have a new batch of this monoclonal Ab in stock? Also, as I told you, the experiment worked well at the beginning (with the first lot# we bought) but not so well with the next lot #. My problem is, before receiving this primary antibody (second order of this same product), I had ordered an extra tube of both primary and secondary Ab because I knew I had many experiments to performed. I haven't received those Ab yet (ordered nearly 2 weeks ago) but they are probably already sent. I want to know if I can send you back those new products, knowing the Ab does not work well.

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Answer

Thank you for getting back to me. If you provide me with the details of your most recent order of your primary and secondary antibodies (order number and date) I will do my best to cancel the order before it is shipped and provide you with credit to the value of the order. If you have received the antibodies on receipt of this e-mail you can send them back in the same packaging that you received the antibodies in and you will be reimbursed (minus a 20% restocking fee). Please return them with the same ice packs that were in the box during shipping (refrozen). I am in contact with the source of this antibody and I will be in touch when I have further information as to the availability of a new batch. I appreciate your patience in this matter.

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Question

Hi, Several weeks ago, I've bought one of your mouse monoclonal antibody (aE11 to C5b-9). As a negative control, I've also bought an isotype control (mouse IgG2a kappa monoclonal (MG2a-53)) and a Goat polyclonal antibody to Mouse IgG PE (ab7002) as a secondary antibody. The purpose of my study is to detect C5b-9 complexes on different cells (lymphocytes, monocytes, etc.) in patients with an inflammatory disease and in controls (by flowcytometry). As positive controls, I activate the complement system by different ways (so we can normally see more C5b-9 on the cells' surface when the complement is activated). At the beginning of my experiment, I've seen many significant differences between the different conditions used (patients, controls and positive controls) whereas now, I can't see those results anymore. The results I obtained with the isotype control are even higher than those with the regular monoclonal Ab; more C5b-9 complexes are present on the cells WITHOUT using the monoclonal antibody. I've noted that this event correlates with the fact that I now use new primary (lot # 166383) and secondary (154790) antibodies. I've received those new Abs less than 3 weeks ago. I first guessed that I might have diluted the secondary Ab more, so I've tried many dilutions (1:5, 1:100, 1:200): No positive results. What dilution of each Ab do you recommended to use in flowcytometry? What do you think the problem could be? Could it be because of the new Abs I use? (Which one?). What do you suggest? Is it possible to send me new Abs (different lot numbers maybe...)? Thank you for your help, Regards,

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Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody, your enquiry definitely sounds batch related. The fact that you have been obtaining negative results in your FACS system until the arrival of the most recent batch suggests that the problem is with the new primary antibody that you have received. Unfortunately at the moment we only have batch # 166383 in stock. However, I would like to offer you a credit note that you can use against the cost of another Abcam antibody. This is provided that the antibody was purchased within the past 90 days. If this is the case please e-mail me the date of purchase and the original purchase order number. I look forward to hearing from you.

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Question

I would kindly ask if it is possible to get a free sample of secondary antibody Cat. No. ab7002. We would like to use this antibody to analyze CK 20 (primary mouse antibody, Dako) in urothelial cells by flow cytometry and immunofluorescence microscopy. Thanking you in advance for your courtesy,

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Answer

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

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Question

Hello. I am interested in your product ab7002-500, goat polyclonal to mouse IgG RPE conjugated, or ab7000-500 (the same but made in rabbit). However, I need the antibody to be adsorbed against rat, so that it doesn't cross-react with rat IgG. You say that you have removed unwanted reactivities but you do not say what reactivities these are. Please confirm what reactivities were removed from these two antibodies Hope you can help. Thanks.

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Answer

ab7002 has minimized cross-reactivity with Bovine, Horse, Human, Rabbit and Swine serum proteins. Ab7000 has minimized cross-reactivity with Human serum proteins. Since rat IgG's are not on the list of either product, the antibodies (anti-Mouse) may quite possibly have non-specific binding with rat IgG.

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Question

How much ab should I use in order to stain 2 x 10 8 cells ? Could you please provide me with some information about molecular weight and concentration of your ab ? Many thanks v. Bernuth

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Answer

This product is 500 kDa if only one antibody is attached and the recommended amount is 1 microgram per 1 X 10(6) cells.

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