Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed (ab5889)

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Key features and details

  • Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt

製品の概要

  • 製品名

    Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, Immunomicroscopy, Flow Cytmore details

  • 吸着処理血清


    Cow, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Mouse IgG F(ab)2 fragment

  • 標識

    PE. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate

    BSA is IgG and protease free
  • Concentration information loading...

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 特記事項（標識）

    R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5889の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/250.
Immunomicroscopy
Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
特記事項
ICC/IF
1/100 - 1/250.
Immunomicroscopy
Use at an assay dependent dilution.

Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab5889 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5889 は 2 報の論文で使用されています。

  • Shi Q  et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). PubMed: 29096715
  • Carocci M  et al. Encephalomyocarditis Virus 2A Protein Is Required for Viral Pathogenesis and Inhibition of Apoptosis. J Virol 85:10741-54 (2011). PubMed: 21849462

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