Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886)

Submit a review Q&A (1)

Key features and details

  • Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy

製品の概要

  • 製品名

    Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • 特異性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgGF(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

  • アプリケーション

    適用あり: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • 吸着処理血清


    Cow, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Mouse IgG F(ab)2 fragment

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 特記事項（標識）

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

関連製品

    アプリケーション

    Our Abpromise guarantee covers the use of ab5886 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    ICC/IF 1/1000 - 1/5000.
    Dot blot Use at an assay dependent dilution.
    WB 1/2000 - 1/10000.
    ELISA 1/20000 - 1/100000.
    IHC Use at an assay dependent dilution.
    Immunomicroscopy Use at an assay dependent dilution.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab5886 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5886 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Hi,



    I'm looking for a biotin labelled F(ab')2 antimouse antibody for peroxidase immunohistochemistry (lung tissue --> first antibody = monoclonal antibody IgG1 that detects adducts of hypoxyprobe). ABCAM provides three different antibodies; ab98657, ab98756 and ab5886. How do these products differ from each other? And what does pre-adsorbed means?



    Many thanks in advance for your help!

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.
    Although, all these three secondary antibodies (ab5886, ab98756, ab98657) recognize mouse IgG and were raised in goat, they are from different sources. The tested application/species/concentration may vary.
    Pre-adsorbed antibodies (ab5886, ab98756) are prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. This means that they only recognize mouse IgG and any cross-reactivity with other species is minimal.
    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com