Goat F(ab) Anti-Rat IgG H&L (Biotin) (ab7176)

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Key features and details

  • Goat F(ab) Anti-Rat IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy

Conjugates logo Related conjugates and formulations

HRP Unconjugated

製品の概要

  • 製品名

    Goat F(ab) Anti-Rat IgG H&L (Biotin)
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Rat

  • 特異性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).

  • アプリケーション

    適用あり: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • 免疫原

    Rat IgG whole molecule

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    IgG and protease free
  • Concentration information loading...

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

  • 特記事項（標識）

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7176の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Dot blot
WB
ELISA
IHC
Immunomicroscopy
特記事項
追加情報
Dot: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/110000.
IHC: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.

This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20000 to 1/110000 of the reconstitution concentration is suggested for this product.

Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab7176 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7176 は 1 報の論文で使用されています。

  • Yang Y  et al. Cryptotanshinone alleviates polycystic ovary syndrome in rats by regulating the HMGB1/TLR4/NF-?B signaling pathway. Mol Med Rep 22:3851-3861 (2020). PubMed: 32901834

レビューと Q&A

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Question

I need a anti-rat biotin conjugated secondary Ab raised in goat to perform IHC on human joint tissue. In addition to ab6275, you have ab6619, 6256, 7176 in your catalog that match that description. Can you tell me if there is any difference between these antibodies and which one you recommend for this application (IHC)?

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Answer

Thank you for your enquiry. Ab6275: Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(ab')2 [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(ab')2. Ab7176: Biotin Conjugated Fab Fragment of Affinity Purified anti-Rat IgG [H&L] [Goat] - This product is the Fab portion (single Fab fragments) of the Goat IgG raised against Rat IgG. Ab6256:Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(c) [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(c). Typically Fab and Fab2 fragments will bind less than the entire IgG molecule. This means these types of products are good for assays in which very low background is desired. I hope I was able to make things more clear. Please do not hesitate to contact me if you have any other questions regarding this issue.

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