Goat F(ab) Anti-Mouse IgG H&L (FITC) (ab6669)
Key features and details
- Goat F(ab) Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISA
Related conjugates and formulations
製品の概要
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製品名
Goat F(ab) Anti-Mouse IgG H&L (FITC)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). -
アプリケーション
適用あり: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details -
免疫原
Mouse IgG whole molecule
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標識
FITC. Ex: 493nm, Em: 528nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項（標識）
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 5.0 moles FITC per mole of Goat IgG Fab -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6669の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Flow Cyt | (1) |
Use at an assay dependent dilution.
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| ELISA |
1/10000 - 1/50000.
|
| 特記事項 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/50000. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab6669 は 4 報の論文で使用されています。
- Ollington B et al. Immunoresponsive Tissue-Engineered Oral Mucosal Equivalents Containing Macrophages. Tissue Eng Part C Methods 27:462-471 (2021). PubMed: 34210153
- Kochegarov A et al. A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos. J Biomed Sci 20:20 (2013). PubMed: 23522091
- Mogilenko DA et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J : (2012). PubMed: 22271762
- Mogilenko DA et al. Modified low density lipoprotein stimulates complement C3 expression and secretion via liver X receptor and toll-like receptor 4 activation in human macrophages. J Biol Chem : (2011). PubMed: 22194611
