Goat F(ab) Anti-Mouse IgG H&L (ab6668)
Key features and details
- Goat F(ab) Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, IHC, ELISA
Related conjugates and formulations
製品の概要
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製品名
Goat F(ab) Anti-Mouse IgG H&L
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC, ELISAmore details -
免疫原
Mouse IgG whole molecule
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).” -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6668の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000.
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ICC/IF |
1/1000 - 1/5000.
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|
IHC |
1/1000 - 1/5000.
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|
ELISA |
1/50000.
|
特記事項 |
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WB
1/2000 - 1/10000. |
ICC/IF
1/1000 - 1/5000. |
IHC
1/1000 - 1/5000. |
ELISA
1/50000. |
画像
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SDS-PAGE results of Goat Fab Anti-Mouse IgG (H&L) ab6668.
Lane 1: Non-reduced Goat Fab Anti-Mouse.
Lane 2: Opal Prestained Ladder.
Lane 3: Reduced Goat Fab Anti-Mouse.
Load: 1.0 µg.
Coomassie stained and imaged.
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Dot Blot results of Goat Fab Anti-Mouse IgG Antibody ab6668.
Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng.
Blocked for 30 min at RT.
Primary Antibody: Goat Fab Anti-Mouse IgG at 1 µg/mL for 1 hr at RT.
Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30 min at RT.
Imaged with BioRad ChemiDoc, Chemi filter.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab6668 は 36 報の論文で使用されています。
- Kaneko H et al. Zoledronate/Anti-VEGF Neutralizing Antibody Combination Administration Increases Osteal Macrophages in a Murine Model of MRONJ Stage 0-like Lesions. J Clin Med 12:N/A (2023). PubMed: 36902701
- Kennedy EA et al. Age-associated features of norovirus infection analysed in mice. Nat Microbiol 8:1095-1107 (2023). PubMed: 37188813
- Kin K et al. Adolescent stress impairs postpartum social behavior via anterior insula-prelimbic pathway in mice. Nat Commun 14:2975 (2023). PubMed: 37221211
- Kokotović T et al. Transcription factor mesenchyme homeobox protein 2 (MEOX2) modulates nociceptor function. FEBS J 289:3457-3476 (2022). PubMed: 35029322
- Xue Y et al. Mechanical tension mobilizes Lgr6+ epidermal stem cells to drive skin growth. Sci Adv 8:eabl8698 (2022). PubMed: 35476447