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  1. Link

    goat-chicken-igy-hl-hrp-preadsorbed-ab7118.pdf

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Secondary antibodies anti-Chicken IgY Enzyme HRP
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Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118)

  • Datasheet
  • SDS
Submit a review Q&A (3)

Product price, shipping and contact information

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こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Goat Anti-Chicken IgY H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Suitable for: WB, Immunomicroscopy, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr

こちらの製品もご検討ください

クロモゲン
Product image
DAB Substrate Kit (ab64238)
ブロッキング
Product image
Normal Goat Serum (ab7481)

関連製品

製品の概要

  • 製品名

    Goat Anti-Chicken IgY H&L (HRP) preadsorbed
    IgY 二次抗体 製品一覧
  • 由来種

    Goat
  • ターゲット生物種

    Chicken
  • アプリケーション

    適用あり: WB, Immunomicroscopy, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Frmore details
  • 吸着処理血清


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    Full length native chicken IgY (purified).

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 特記事項(精製)

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)

    Horseradish Peroxidase (HRP)
  • ポリ/モノ

    ポリクローナル
  • 研究分野

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Enzyme
    • HRP

関連製品

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

アプリケーション

Our Abpromise guarantee covers the use of ab7118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/2500.
Dot blot Use at an assay dependent dilution.
ELISA 1/20000 - 1/100000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (0)

    ab7118 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7118 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Where is the questionnaire that I should fill out? If it is the note with product number and lot number, I already filled that out in my previous inquiry.

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2012

    Answer

    Thank you for your quick response.

    The Questionnaire has been attached to my first response as a word document but I have reattached it to this e-mail as well.

    Please could you add further information to the Questionnaire? I am particularly interested in sample preparation, lysis buffer. Have you run a secondary antibody ONLY control (without the sample and primary antibody)? Liver cells/tissues have very high concentration of intracellular proteins. It may be worth increasing the concentration of the blocking agent from 5-10%. Have you had any chance to use BSA rather than milk for blocking?

    Could you please confirm the Abcam Order number and the arrival date as well?

    I look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    Answered on Oct 12 2012

    Question

    1) Abcam product code ab7118



    2) Abcam order reference number or product batch number lot nr GR27997-6



    3) Description of the problem: unspecific bands on western, with using only the secondary antibody (no primary)



    4) Sample preparation:

    Type of sample (whole cell lysates, fraction, recombinant protein…): tissue homogenates, centrifuged 4000xg, supernatants dissolved in Laemli

    Lysis buffer: Laemli (SDS, DTT)

    Protease inhibitors: Pefabloc (SDigma-Aldrich) + Leupeptin

    Phosphatase inhibitors: no

    Reducing agent: DTT

    Boiling for ≥5 min? yes/no: 65C 15 min

    Protein loaded ug/lane or cells/lane: approximately 10-30 ug protein per lane

    Positive control

    Negative control



    5) Percentage of gel: 4-15% polyacrylamide

    Type of membrane: PVDF

    Protein transfer verified: the marker, which is blue is gone from the gel, and can be seen on the membrane

    Blocking agent and concentration: milk 5%

    Blocking time: 1 h

    Blocking temperature: RT



    6) Primary antibody (If more than one was used, describe in “additional notes”) :

    NO PRIMARY ANTIBODY!



    7) Secondary antibody:

    Species: goat

    Reacts against: chicken

    Concentration or dilution: 1:5000

    Diluent buffer: PBS-T

    Incubation time: 1 h

    Incubation temperature: RT

    Fluorochrome or enzyme conjugate: HRP



    8) Washing after primary and secondary antibodies:

    Buffer PBS-T

    Number of washes: 4-5



    9) Detection method: ECL



    10) How many times have you run this staining? Two times, with different samples, because I thought first that something was wrong with my samples.

    Do you obtain the same results every time? yes

    What steps have you altered to try and optimize the use of this antibody? Not optimized, I would like hear first what your suggestions are.



    Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.







    From: technical@abcam.com [mailto:technical@abcam.com]
    Sent: 12. oktober 2012 09:32
    To: Aleksandra Rojek
    Subject: Reply from Abcam to your enquiry regarding ab7118 [CCE4241379]



















    https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









    Dear Dr Rojek

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

    Please could you provide some further details of the protocol used and complete the following form (attached as a word document).

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.




    Best regards,
    Tanya

    Tanya Bagrij, PhD
    Scientific Support Supervisor
    Abcam plc
    www.abcam.com


    Your original inquiry to Abcam:


    Lot number: GR27997-6

    Enquiry: Hi, I experience problems with the secondary antibody: I see bands on the blot, without adding primary antibody. This would indicate unspecific binding of secondary antibody to the proteins on the membrane. How do I avoid unspecific binding of secondary ab? Is there any way of more stringent washing (protocol)? WB conditions: 20 ul mouse liver homogenate per lane, blocking 5% milk, detection ECL, 4-15% polyacrylamide gel, dry transfer, secondary 1:5000 diluted in PBS-T 1hr RT, washes 4-5 times 5-15 min. (This protocol worked fine previously with different primary ab made in rabbit, and goat anti rabbit secondaries, and we have not previously experienced unspecific signals from secondary alone) Best regards,




    Abcam Customer Services and Scientific Support Team
    https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

    [CCE4241379]













    Discover more at abcam.com

    Read More

    Abcam community

    Verified customer

    Asked on Oct 15 2012

    Answer

    Thank you for your enquiry regardingab7118 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.

    - Have you run a secondary antibody ONLY control (without the sample and primary antibody)?

    - Liver cells/tissues have very high concentration of intracellular proteins. Have you tried loading 5 or ug per lane?

    - It may be worth increasing the concentration of the blocking agent from 5-10%. Have you had any chance to use BSA rather than milk for blocking?

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

    Read More

    Abcam Scientific Support

    Answered on Oct 15 2012

    Question

    Lot number: GR27997-6
    Enquiry: Hi, I experience problems with the secondary antibody: I see bands on the blot, without adding primary antibody. This would indicate unspecific binding of secondary antibody to the proteins on the membrane. How do I avoid unspecific binding of secondary ab? Is there any way of more stringent washing (protocol)? WB conditions: 20 ul mouse liver homogenate per lane, blocking 5% milk, detection ECL, 4-15% polyacrylamide gel, dry transfer, secondary 1:5000 diluted in PBS-T 1hr RT, washes 4-5 times 5-15 min. (This protocol worked fine previously with different primary ab made in rabbit, and goat anti rabbit secondaries, and we have not previously experienced unspecific signals from secondary alone) Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2012

    Answer

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

    Please could you provide some further details of the protocol used and complete the following form (attached as a word document).

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

    Read More

    Abcam Scientific Support

    Answered on Oct 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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