Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiac fibroblasts)
Loading amount
4 µg
Specification
Cardiac fibroblasts
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Abcam antibody:Goat anti-Chicken IgY H&L (HRP) secondary antibody
Dilution
1/2500
Detection
Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 57 kDa
Additional data
Additional Notes
Excellent antibody for detection of mouse Vimentin by Western blot. Do not give unspecific bands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 04 2013