Goat Anti-Chicken IgY H&L (HRP) (ab97135)
Key features and details
- Goat Anti-Chicken IgY H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
製品の概要
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製品名
Goat Anti-Chicken IgY H&L (HRP)
IgY 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Chicken -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項(標識)
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
関連製品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97135の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC |
Use at an assay dependent dilution.
|
|
IHC-P |
1/200 - 1/5000.
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|
ELISA | (1) |
1/10000 - 1/100000. (Primary).
|
WB | (1) |
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000
|
特記事項 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (Primary). |
WB
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000 |
画像
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All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 90 seconds -
All lanes : Anti-Vimentin antibody (ab24525)
All lanes : Mouse cardiac fibroblasts
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab97135) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 seconds
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Ab97135 used to detect a chicken anti-beta Galactosidase antibody (ab9361). Ab97135 used at a concentration of 1:2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands: 70 Kda. NB. Gels run on 10% Acrylamide gels.
This image is courtesy of an anonymous Abreview.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab97135 は 36 報の論文で使用されています。
- Fazal SV et al. SARM1 detection in myelinating glia: sarm1/Sarm1 is dispensable for PNS and CNS myelination in zebrafish and mice. Front Cell Neurosci 17:1158388 (2023). PubMed: 37091921
- Kang SG et al. Innate Immune Status of Glia Modulates Prion Propagation in Early Stage of Infection. Cells 12:N/A (2023). PubMed: 37508542
- Enders L et al. Pharmacological perturbation of the phase-separating protein SMNDC1. Nat Commun 14:4504 (2023). PubMed: 37587144
- Hines MR et al. Extracellular biomolecular free radical formation during injury. Free Radic Biol Med 188:175-184 (2022). PubMed: 35724853
- Liu CW et al. Blood-Brain Barrier Disruption in Preclinical Mouse Models of Stroke Can Be an Experimental Artifact Caused by Craniectomy. eNeuro 9:N/A (2022). PubMed: 36224001