Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)

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Key features and details

  • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

Conjugates logo Related conjugates and formulations

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製品の概要

  • 製品名

    Goat Anti-Chicken IgY H&L (Biotin) preadsorbed
    IgY 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Chicken

  • アプリケーション

    適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 吸着処理血清


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native chicken IgY (purified).

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    IgY fraction

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7117の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
特記事項
追加情報
Dot: Use at an assay dependant dilution.
ELISA: 1/8000 - 1/36000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab7117 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7117 は論文での使用が確認できていません。

レビューと Q&A

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Question

Thanks for your help, but I am still uncertain about my question regarding the addition of glycerol. "The storage instructions stated to add 50% glycerol before freezing. Should this be 50% of the current volume or a final concentration of 50%".

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Answer

Thank you for your message. I have double-checked with my colleagues and actually we have reconstituted this antibody and already added 50% glycerol to the buffer. This means that for long term storage, you do not need to add anything just aliquot the product and keep it at -20°C. We have corrected our on-line datasheet in order to avoid more confusion. Thank you again for bringing this matter to our attention.

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