Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)

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Key features and details

  • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

製品の概要

  • 製品名

    Goat Anti-Chicken IgY H&L (Biotin) preadsorbed
    IgY 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Chicken

  • アプリケーション

    適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 吸着処理血清


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native chicken IgY (purified).

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    IgY fraction

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • 追加情報
    Dot: Use at an assay dependant dilution.
    ELISA: 1/8000 - 1/36000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab7117 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7117 は論文での使用が確認できていません。

    レビューと Q&A

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    Question

    Thanks for your help, but I am still uncertain about my question regarding the addition of glycerol. "The storage instructions stated to add 50% glycerol before freezing. Should this be 50% of the current volume or a final concentration of 50%".

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    Answer

    Thank you for your message. I have double-checked with my colleagues and actually we have reconstituted this antibody and already added 50% glycerol to the buffer. This means that for long term storage, you do not need to add anything just aliquot the product and keep it at -20°C. We have corrected our on-line datasheet in order to avoid more confusion. Thank you again for bringing this matter to our attention.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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