Goat Anti-Chicken IgY H&L (Biotin) (ab207998)
Key features and details
- Goat Anti-Chicken IgY H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, ICC/IF, WB, Flow Cyt, IP, IHC-Fr, IHC-P
製品の概要
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製品名
Goat Anti-Chicken IgY H&L (Biotin)
IgY 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Chicken -
アプリケーション
適用あり: ELISA, ICC/IF, WB, Flow Cyt, IP, IHC-Fr, IHC-Pmore details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab207998の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/20000 - 1/200000.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
1/200 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ELISA
1/20000 - 1/200000. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
1/200 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
画像
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IHC image of Cytokeratin 8 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. Ab207998 Goat Anti-Chicken IgY H & L (Biotin) was used as the secondary antibody.
Staining was performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab107115, 1/100 dilution, for 15 mins at room temperature, followed by ab207998, 1/200 dilution, for 15 mins at room temperature. Detection was via an HRP conjugated ABC system and DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ab207998 was tested by direct ELISA, where wells were coated with serially diluted chicken IgY (1000 – 16 ng/ml) for 2 hours, followed by a 2 hour blocking step (5% BSA). ab207998 (1:20,000 dilution; 2 hours) was added and detected by streptavidin-HRP (ab7403; 1:10,000 dilution; 1 hour). Signal was developed by TMB substrate. Data from duplicates; +/- SD.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab207998 は 1 報の論文で使用されています。
- Hutton C et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell 39:1227-1244.e20 (2021). PubMed: 34297917