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Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (ab7119)

Submit a review Q&A (1)

Key features and details

  • Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed
  • Conjugation: Alkaline Phosphatase
  • Host species: Goat
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

製品の概要

  • 製品名

    Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed
    IgY 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Chicken

  • アプリケーション

    適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 吸着処理血清


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native chicken IgY (purified).

  • 標識

    Alkaline Phosphatase

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads.

  • 特記事項（標識）

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7119の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
特記事項
追加情報
Dot: Use at an assay dependant dilution.
ELISA: 1/1000 - 1/4000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab7119 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7119 は論文での使用が確認できていません。

レビューと Q&A

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Question

Could you tell me the difference between ab7119 and ab6878 in immunoblotting examination?　My target molecules are from ruminants including bovine.　From your data sheet I should use ab7119 rather than ab6878 due to lower background.　Please give me your suggestion.

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Answer

Thank you for your inquiry. I can confirm that both recognize whole chicken IgY and IgG (H+L).　However, ab7119 has been cross adsorbed to remove reactivity with bovine,　goat, guinea pig, hamster, horse, human, mouse, rabbit, rat and sheep serum　proteins. Meanwhile, ab6878 has not been cross adsorbed. Therefore, I would suggest using ab7119 instead as it will have less cross reactivity with bovine. I hope this information helps. If there is anything else that I can help you with, please do not hesitate to contact me.

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