Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (ab175675)
Key features and details
- Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
製品の概要
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製品名
Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed
IgY 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Chicken -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
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アプリケーション
適用あり: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
吸着処理血清
Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Alexa Fluor® 405. Ex: 402nm, Em: 421nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. The antibody to chicken IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175675の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
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| 特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
画像
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (ab175675)
ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175675, Alexa Fluor® 405 Goat polyclonal Secondary Antibody to Chicken IgY - H&L pre-adsorbed, used at 1µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (ab175675)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (15)
ab175675 は 15 報の論文で使用されています。
- Lindhout FW et al. Centrosome-mediated microtubule remodeling during axon formation in human iPSC-derived neurons. EMBO J 40:e106798 (2021). PubMed: 33835529
- Cao Y et al. Microtubule Minus-End Binding Protein CAMSAP2 and Kinesin-14 Motor KIFC3 Control Dendritic Microtubule Organization. Curr Biol 30:899-908.e6 (2020). PubMed: 32084403
- De Pace R et al. Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons. Cell Rep 31:107775 (2020). PubMed: 32553155
- Ly C et al. Bryostatin 1 Promotes Synaptogenesis and Reduces Dendritic Spine Density in Cortical Cultures through a PKC-Dependent Mechanism. ACS Chem Neurosci 11:1545-1554 (2020). PubMed: 32437156
- Vevea JD & Chapman ER Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. Elife 9:N/A (2020). PubMed: 32515733
