Anti-GNLY/Granulysin 抗体 [EPR22110-101] (ab241333)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22110-101] to GNLY/Granulysin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GNLY/Granulysin antibody [EPR22110-101]
GNLY/Granulysin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22110-101] to GNLY/Granulysin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged human GNLY/Granulysin recombinant protein IHC-P: Human endometrium and spleen tissue. ICC/IF: Human PBMC cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22110-101 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab241333の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. |
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WB |
1/1000. Predicted molecular weight: 16 kDa.
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ICC/IF |
1/250.
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特記事項 |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. |
WB
1/1000. Predicted molecular weight: 16 kDa. |
ICC/IF
1/250. |
ターゲット情報
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機能
Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. -
組織特異性
Expressed in natural killer and T-cells. -
配列類似性
Contains 1 saposin B-type domain. -
翻訳後修飾
A 9 kDa form is produced by proteolytic processing of a 15 kDa protein. -
細胞内局在
Secreted. Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation. - Information by UniProt
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参照データベース
- Entrez Gene: 10578 Human
- Omim: 188855 Human
- SwissProt: P22749 Human
- Unigene: 105806 Human
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別名
- 519 antibody
- D2S69E antibody
- GNLY antibody
see all
画像
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PBMC (human peripheral blood mononuclear cell) cells labeling GNLY/Granulysin with ab241333 at 1/250 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) (Green) at 1/1000 dilution.
Confocal image showing cytoplasmic staining in a part of human PBMC. The nuclear counterstain was DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain (Red).
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Anti-GNLY/Granulysin antibody [EPR22110-101] (ab241333) at 1/1000 dilution + His-tagged human GNLY/Granulysin recombinant protein (aa23-145) 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 16 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds
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Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling GNLY/Granulysin with ab241333 at 1/4000 dilution, followed by a ready to use Goat anti-Rabbit IgG H&L (HRP). The section was incubated with ab for 30 mins at 37°C. Sporadic staining on human spleen (PMID: 30151671) is observed, performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins.
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Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling GNLY/Granulysin with ab241333 at 1/4000 dilution, followed by a ready to use Goat anti-Rabbit IgG H&L (HRP). The section was incubated with ab for 30 mins at 37°C. Positive staining on lymphocytes of human endometrium (PMID: 26488136) is observed, performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab241333 は 1 報の論文で使用されています。
- Liu X et al. Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma. Nat Commun 13:1158 (2022). PubMed: 35241665