Anti-GNB3 抗体 [EPR8862] (ab154866)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8862] to GNB3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GNB3 antibody [EPR8862]
GNB3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8862] to GNB3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, A673 cell lysate; Human fetal brain tissue lysate; Human fetal lung tissue lysate; Human kidney tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR8862 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154866の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. -
配列類似性
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats. - Information by UniProt
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参照データベース
- Entrez Gene: 2784 Human
- Entrez Gene: 14695 Mouse
- Entrez Gene: 60449 Rat
- Omim: 139130 Human
- SwissProt: P16520 Human
- SwissProt: Q61011 Mouse
- SwissProt: P52287 Rat
- Unigene: 631657 Human
see all -
別名
- G protein beta 3 subunit antibody
- GBB3_HUMAN antibody
- GNB3 antibody
see all
画像
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All lanes : Anti-GNB3 antibody [EPR8862] (ab154866) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : A673 cell lysate
Lane 4 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GNB3 with ab154866 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human retina tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154866 は 2 報の論文で使用されています。
- Wang Q et al. Identification of RyR2-PBmice and the effects of transposon insertional mutagenesis of the RyR2 gene on cardiac function in mice. PeerJ 7:e6942 (2019). PubMed: 31143551
- McGlinchey JC et al. Correction of the Pathogenic Alternative Splicing, Caused by the Common GNB3 c.825C>T Allele, Using a Novel, Antisense Morpholino. Nucleic Acid Ther 26:257-65 (2016). PubMed: 27028457