Key features and details
- Rabbit polyclonal to GNB1
- Suitable for: WB
- Reacts with: Rat, Sheep, Human
- Isotype: IgG
GNB1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to GNB1
アプリケーション適用あり: WBmore details
種交差性交差種: Rat, Sheep, Human
交差が予測される動物種: Dog, Xenopus laevis, Zebrafish, Amphibian, Chinese hamster
- WB: sheep retinal extracts, rat brain, Jurkat, MC7, MDA-MB-231, T-47D
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
精製度Immunogen affinity purified
一次抗体 備考Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
The Abpromise guarantee
Use a concentration of 2 µg/ml.
Use a concentration of 2 µg/ml.
機能Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
配列類似性Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
翻訳後修飾Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.
- Information by UniProt
- Beta subunit signal transducing proteins GS/GI antibody
- G protein beta 1 subunit antibody
- GBB1 antibody
All lanes : Anti-GNB1 antibody (ab3433) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 5 : tissue extract of Rat Brain
Lysates/proteins at 30 µg per lane.
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Additional bands at: ~37 kDa. We are unsure as to the identity of these extra bands.
All lanes : Anti-GNB1 antibody (ab3433) at 2 µg/ml
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with skimmed milk
Lane 2 : Rat Brain with skimmed milk
Blocking peptides at 5 % per lane.
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Western blot of Tr beta on sheep retinal extracts using ab3433. Western blot of GNB1 on sheep retinal extracts using ab3433.