Anti-GNB1 抗体 (ab3433)
Key features and details
- Rabbit polyclonal to GNB1
- Suitable for: WB
- Reacts with: Rat, Sheep, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GNB1 antibody
GNB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GNB1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Sheep, Human
交差が予測される動物種: Dog, Xenopus laevis, Zebrafish, Amphibian, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human GNB1 aa 8-25.
Sequence:RQEAEQLKNQIRDARKAC
(Peptide available asab4972) -
ポジティブ･コントロール
- WB: sheep retinal extracts, rat brain, Jurkat, MC7, MDA-MB-231, T-47D
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3433の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. -
配列類似性
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats. -
翻訳後修飾
Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP. - Information by UniProt
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参照データベース
- Entrez Gene: 2782 Human
- Entrez Gene: 24400 Rat
- Entrez Gene: 322104 Zebrafish
- Omim: 139380 Human
- SwissProt: P62873 Human
- SwissProt: P54311 Rat
- SwissProt: P79959 Xenopus laevis
- SwissProt: Q6PH57 Zebrafish
see all -
別名
- Beta subunit signal transducing proteins GS/GI antibody
- G protein beta 1 subunit antibody
- GBB1 antibody
see all
画像
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Western blot - Anti-GNB1 antibody (ab3433)All lanes : Anti-GNB1 antibody (ab3433) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 5 : tissue extract of Rat Brain
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Additional bands at: ~37 kDa. We are unsure as to the identity of these extra bands.Detection: chemiluminescence.
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Western blot - Anti-GNB1 antibody (ab3433)All lanes : Anti-GNB1 antibody (ab3433) at 2 µg/ml
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with skimmed milk
Lane 2 : Rat Brain with skimmed milk
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilutionDetection: chemiluminescence.
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Western blot - Anti-GNB1 antibody (ab3433)Western blot of Tr beta on sheep retinal extracts using ab3433. Western blot of GNB1 on sheep retinal extracts using ab3433.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab3433 は論文での使用が確認できていません。
