Anti-GNAQ 抗体 [EPR17149] (ab199533)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17149] to GNAQ
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GNAQ antibody [EPR17149]
GNAQ 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17149] to GNAQ -
由来種
Rabbit -
特異性
The immunogen used for this product shares 77% homology with GNA11. Cross-reactivity with this protein has not been confirmed experimentally. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HEK293, HeLa, HepG2, C6, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, mouse kidney, mouse spleen, rat brain and rat kidney lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17149 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab199533の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
ターゲット情報
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機能
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. -
組織特異性
Predominantly expressed in ovary, prostate, testis and colon. -
配列類似性
Belongs to the G-alpha family. G(q) subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 2776 Human
- Entrez Gene: 14682 Mouse
- Entrez Gene: 81666 Rat
- Omim: 600998 Human
- SwissProt: P50148 Human
- SwissProt: P21279 Mouse
- SwissProt: P82471 Rat
- Unigene: 269782 Human
see all -
別名
- CMC1 antibody
- G alpha Q antibody
- G protein alpha Q antibody
see all
画像
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All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : C6 (Rat glial tumor cells) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab199533 は 2 報の論文で使用されています。
- Randolph CE et al. Enhanced membrane binding of oncogenic G protein αqQ209L confers resistance to inhibitor YM-254890. J Biol Chem 298:102538 (2022). PubMed: 36174676
- Yokoi N et al. Identification of PSD-95 Depalmitoylating Enzymes. J Neurosci 36:6431-44 (2016). PubMed: 27307232