Anti-GNAI1 + GNAI2 抗体 (ab3522)

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Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (ab3522)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (ab3522)

Key features and details

  • Rabbit polyclonal to GNAI1 + GNAI2
  • Suitable for: IHC-P, ICC
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-GNAI1 + GNAI2 antibody

  • 製品の詳細

    Rabbit polyclonal to GNAI1 + GNAI2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, ICCmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Chicken, Guinea pig, Hamster, Cow, Dog, Xenopus laevis, Starfish

  • 免疫原

    Synthetic peptide corresponding to Human GNAI1 + GNAI2 aa 300-400.
    Database link: P63096
    (Peptide available as ab5006)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • IHC-P: Human tonsil tissue. ICC: SH-SY5Y cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 一次抗体 備考

    The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3522の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use a concentration of 2 µg/ml.
特記事項
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.

  • 配列類似性

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.

  • 細胞内局在

    Nucleus. Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Adenylate cyclase-inhibiting G alpha protein antibody
    • Gi antibody
    • Gi1 protein alpha subunit antibody
    • Gi2 protein alpha subunit antibody
    • GIP antibody
    • GNAI1 antibody
    • GNAI1_HUMAN antibody
    • GNAI2 antibody
    • GNAI2B antibody
    • Guanine nucleotide binding protein G(i) alpha 1 subunit antibody
    • Guanine nucleotide binding protein G(i) alpha 2 subunit antibody
    • Guanine nucleotide-binding protein G(i) subunit alpha-1 antibody
    see all

画像

  • Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (ab3522)
    Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (ab3522)

    SH-SY5Y cells stained for GNAI1 (green) using ab3522 at 2 µg/mL in immunocytochemical analysis. The nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI, F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel e shows the no primary antibody control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (ab3522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (ab3522)

    IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献 (4)

ab3522 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3522 は 4 報の論文で使用されています。

  • Vosahlikova M  et al. Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of d-opioid receptor amount and function. Biochem Pharmacol 154:452-463 (2018). PubMed: 29883706
  • Cechova K  et al. Up-regulation of µ-, d- and ?-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes. J Neuroimmunol 321:12-23 (2018). PubMed: 29957382
  • Doi M  et al. Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour. Nat Commun 7:10583 (2016). WB . PubMed: 26882873
  • Ufer C & Germack R Cross-regulation between beta1- and beta3-adrenoceptors following chronic beta-adrenergic stimulation in neonatal rat cardiomyocytes. Br J Pharmacol 158:300-13 (2009). WB ; Rat . PubMed: 19719783

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