Anti-GM-CSF 抗体 (ab9741)

Submit a review Q&A (5)References (4)

Key features and details

  • Rabbit polyclonal to GM-CSF
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Human
  • Isotype: unknown

製品の概要

  • 製品名

    Anti-GM-CSF antibody
    GM-CSF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to GM-CSF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Highly pure (>98%) recombinant mGM-CSF (mouse Granulocyte Macrophage Colony Stimulating Factor)

  • 特記事項

    Although some customers have been successful in IHC we no longer batch test in this application.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.2 µg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

ターゲット情報

  • 機能

    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

  • 配列類似性

    Belongs to the GM-CSF family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Colony stimulating factor 2 (granulocyte-macrophage) antibody
      • Colony Stimulating Factor 2 antibody
      • Colony stimulating factor antibody
      • Colony-stimulating factor antibody
      • CSF 2 antibody
      • CSF antibody
      • CSF2 antibody
      • CSF2_HUMAN antibody
      • GM-CSF antibody
      • GMCSF antibody
      • Granulocyte Macrophage Colony Stimulating Factor antibody
      • Granulocyte-macrophage colony-stimulating factor antibody
      • MGC131935 antibody
      • MGC138897 antibody
      • MGI1GM antibody
      • Molgramostin antibody
      • Pluripoietin-a antibody
      • Sargramostim antibody
      see all

    画像

    • Western blot - Anti-GM-CSF antibody (ab9741)
      Western blot - Anti-GM-CSF antibody (ab9741)

      Western blot analysis of recombinant mGM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.

    • Western blot - Anti-GM-CSF antibody (ab9741)
      Western blot - Anti-GM-CSF antibody (ab9741)

      Western blot analysis of recombinant mGM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.

    • ELISA - Anti-GM-CSF antibody (ab9741)
      ELISA - Anti-GM-CSF antibody (ab9741)
      Less than 0.10 ug/ml ab9741 will neutralize 50% of the bioactivity of 1 ng/ml m-GM-CSF.

    参考文献 (4)

    ab9741 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9741 は 4 報の論文で使用されています。

    • Liu H  et al. Decreased expression of granulocyte-macrophage colony-stimulating factor is associated with adverse clinical outcome in patients with gastric cancer undergoing gastrectomy. Oncol Lett 14:4701-4707 (2017). PubMed: 28943964
    • Bayih AG  et al. Immune Response and Protective Efficacy of a Heterologous DNA-Protein Immunization withLeishmaniaSuperoxide Dismutase B1. Biomed Res Int 2017:2145386 (2017). WB ; Mouse . PubMed: 29359145
    • Siwetz M  et al. TNF-a alters the inflammatory secretion profile of human first trimester placenta. Lab Invest N/A:N/A (2016). PubMed: 26752743
    • Chung I  et al. Inhibitory mechanism of Korean Red Ginseng on GM-CSF expression in UVB-irradiated keratinocytes. J Ginseng Res 39:322-30 (2015). PubMed: 26869824

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    Question

    Inquiry: Dear Sir/ Madam, Sorry to disturb you. Our customer raised an enquiry about ab9741. She already used ab9741 to conduct the ICC/IF assay with primary culture cell--Rat Cerebral Cortex tissue. She thinks the expression of GM-CSF protein should in the nuclear and cytosol of her sample. But the result shows the specific signal just in the nuclear. Besides, she also wants to know is there any customer conducted the same experiment just like her or not? If yes, could you please offer any suggestions to improve her results? Furthermore, could you please offer any selections which can be a positive control in her experiment? Please help this customer to solve these problems? Thank you very much. Best regards, Ling

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    Answer

    Thank you for contacting us.

    ab9741 has not been tested in ICC/IF so we do not have any useful information to provide the customer.

    Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    Dear Tech support

    One of our customers is interested in inhibiting cytokine mediated immune responses in Human and Mouse cell lines.
    The customer has asked the following questions regarding products ab9741, ab64715:

    What is the difference between neutralizing and inhibition functions stated in Abcams datasheets.
    The datasheet also states the required antibody concentration for a 50% inhibition of bioactivity, wouldan increase inconcentration lead to a linear increase in inhibition?

    Thank you for your kind help.
    Regards,

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    Answer

    Thank you for contacting us and sorry for the delay in getting back to you.

    The neutralizing and inhibition functions stated on the datasheet of the antibodies ab9741 andab64715 relate to the assays which have been performed to validate their activities.

    With the Anti-GM-CSF antibody (ab9741), the antibody has been used in a neutralising assay as described in the protocol attached to this email.

    Whilst with the Anti-TGF beta 1 antibody [2Ar2] (ab64715) has been used in an inhibition of proliferation assay. This was carried out asdescribed in Tsang, M. et al., (1995) Cytokine 7:389.The dose response curves are given in figures 1 and 2 of the attached document.The response is linear over a small range.

    I hope this information has been of help. If you require any further information, please do not hesitate to ask.

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    Question


    Inquiry: Dear Sir/ Madam, Our customer raise an inquiry about Anti-GM-CSF antibody (ab9741). She would like to test in Rat/ WB. Besides, she would like to know the percentage of sequence homology between rat sequence and mouse immunogen.
    Would you please help her create the discount code and provide the information?
    The information of this customer is:
    Beat regards,

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    Answer

    Thank you very much for your inquiry.

    The immunogen of ab9741 was the whole GM-CSFprotein of mouse. Mouse GM-CSF only shows 71% homology with rat as a whole protein and therefore I cannot recommend to use this antibody for rat samples.

    We do have two antibodies that have a immunogen (peptide) that is taken from a part of the GM-CSF human or ratsequence that shows a higher homology to the rat sequence. Both antibodies are predicted to react with rat and I would be happy to issue a discount code for either of them.

    Maybe your customer would like to review the datasheets and choose one of them for testing in rat?

    ab77768

    https://www.abcam.com/index.html?datasheet=77768 (or use the following: https://www.abcam.com/index.html?datasheet=77768).

    ab114987

    https://www.abcam.com/index.html?datasheet=114987 (or use the following: https://www.abcam.com/index.html?datasheet=114987).

    Please let me know if your customer would like to take this offer and I will be happy to issue a testing discount code for them.

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    Question

    Dear Sir/Madam, I want to know antibody(ab9741) in WB experiments should be location at which what molecular weight?

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    Answer

    Thank you for contacting us with your question.
    The GM-CSF protein is expected to run around 16 kDa, however there are 4 known glycosylation sites, each adding around 2 kDa to the protein, so the protein generally runs around 24 kDa on a Western blot.
    I hope this information will be useful, but if you have any further questions or if there is anything else we can do for you, please let me know and I'll be happy to help.

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    Question

    ANTIBODY CODE ab9741 BATCH NUMBER 19349 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands, we are finding bands at just above 20, and two bands between 37-50 kD. these higher molecular weight bands appear to be differentially expressed in our LPS treated tissues compared to control tissues. Do you have any information on these bands? thank you, SAMPLE total protein from uterine and cervical tissues (mouse) PRIMARY ANTIBODY abcam gmcsf SECONDARY ANTIBODY anti-rabbit DETECTION METHOD ECL PLus ANTIBODY STORAGE CONDITIONS 4 degrees SAMPLE PREPARATION TPER AMOUNT OF PROTEIN LOADED 40 micrograms ELECTROPHORESIS/GEL CONDITIONS reducing, 12% gel TRANSFER AND BLOCKING CONDITIONS routine HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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    Answer

    Unfortunately, we only test against the recombinant murine GM-CSF and do not test tissue samples. In the naturally occurring GM-CSF there are potential glycolisation sites which could cause the protein to migrate higher on a WB. Sorry we could not be of more assistance on this one.

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