Anti-Glycophorin C/GPC 抗体 [EPR4116] (ab108925)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling Glycophorin C/GPC with Purified ab108925 at 1:1500 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Glycophorin C/GPC with Purified ab108925 at 1:1500 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell, Left) / K-562 (Human chronic myelogenous leukemia lymphoblast, Right) cells labeling Glycophorin C/GPC with purified ab108925 at 1/10000 dilution (0.1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Negative control: A549.

Glycophorin C/GPC was immunoprecipitated from 0.35 mg TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 µg with 108925 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 µg

Lane 2: ab108925 IP in TF-1 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab108925 in TF-1 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Lower band could be N-Deglycosylated GPC. (PMID: 16461900)