Anti-Glycogenin 1 抗体 (ab272606)

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Western blot - Anti-Glycogenin 1 antibody (ab272606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogenin 1 antibody (ab272606)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glycogenin 1 antibody (ab272606)

Key features and details

  • Rabbit polyclonal to Glycogenin 1
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Glycogenin 1 antibody

  • 製品の詳細

    Rabbit polyclonal to Glycogenin 1

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment corresponding to Human Glycogenin 1 aa 200-350.
    Database link: P46976

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • ICC/IF: U-251 MG. WB: Human cell line RT4. IHC-P: Human testis tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
IHC-P
Human
WB
Human
アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
特記事項
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.

ターゲット情報

  • 機能

    Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.

  • パスウェイ

    Glycan biosynthesis; glycogen biosynthesis.

  • 関連疾患

    Defects in GYG1 are the cause of glycogen storage disease type 15 (GSD15) [MIM:613507]. It is a metabolic disorder resulting in muscle weakness, associated with the glycogen depletion in skeletal muscle, and cardiac arrhythmia, associated with the accumulation of abnormal storage material in the heart. The skeletal muscle shows a marked predominance of slow-twitch, oxidative muscle fibers and mitochondrial proliferation.

  • 配列類似性

    Belongs to the glycogenin family.

  • 翻訳後修飾

    Self-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195.
    Phosphorylated.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Glycogenin antibody
      • Glycogenin-1 antibody
      • Glycogenin1 antibody
      • GLYG_HUMAN antibody
      • GN-1 antibody
      • GN1 antibody
      • GYG 1 antibody
      • GYG antibody
      • GYG1 antibody
      see all

    画像

    • Western blot - Anti-Glycogenin 1 antibody (ab272606)
      Western blot - Anti-Glycogenin 1 antibody (ab272606)
      Lane 2 : Anti-Glycogenin 1 antibody (ab272606) at 0.4 µg/ml

      Lane 1 : Molecular weight ladder
      Lane 2 : Human cell line RT4
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogenin 1 antibody (ab272606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogenin 1 antibody (ab272606)

      Paraffin-embedded formalin-fixed Human testis tissue stained for Glycogenin 1 using ab272606 at 1/500 in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-Glycogenin 1 antibody (ab272606)
      Immunocytochemistry/ Immunofluorescence - Anti-Glycogenin 1 antibody (ab272606)

      PFA fixed, Triton X-100 permeablized human cell line U-251 MG labeling Glycogenin 1 (green) using ab272606at 2 ug/ml in ICC/IF analysis.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272606 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272606 は論文での使用が確認できていません。

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