Key features and details
- Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S640)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品名Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody
Glycogen synthase 1/GYS1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S640)
特異性This phospho specific polyclonal antibody is specific for phosphorylated Ser 640 of human muscle glycogen synthase (GYS1). Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.
アプリケーション適用あり: IHC-P, WBmore details
種交差性交差種: Mouse, Rat, Human
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
This product was previously labelled as Glycogen synthase 1
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
一次抗体 備考Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab2479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.
機能Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
パスウェイGlycan biosynthesis; glycogen biosynthesis.
関連疾患Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
配列類似性Belongs to the glycosyltransferase 3 family.
- Information by UniProt
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479) at 1/1000 dilution
Lanes 1 & 5 : mock treated mouse cardiac myocyte lysate at 12 µl
Lane 2 : insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 3 : insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 4 : insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 6 : CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µl
Lane 7 : CLA treated (20 nM, 45min) mouse cardiac myocyte lysate
Lane 8 : CLA treated (100 nM, 45min) mouse cardiac myocyte lysate
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Rabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.A 4-20% Criterion gel for SDS-PAGE was used.
ab2479 は 4 報の論文で使用されています。
- Eckle T et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). PubMed: 22504483
- Ambacher KK et al. The JNK- and AKT/GSK3ß- signaling pathways converge to regulate Puma induction and neuronal apoptosis induced by trophic factor deprivation. PLoS One 7:e46885 (2012). PubMed: 23056511
- Singh V et al. Somatostatin receptor subtype-2-deficient mice with diet-induced obesity have hyperglycemia, nonfasting hyperglucagonemia, and decreased hepatic glycogen deposition. Endocrinology 148:3887-99 (2007). PubMed: 17525126
- Alemzadeh R & Tushaus K Diazoxide attenuates insulin secretion and hepatic lipogenesis in zucker diabetic fatty rats. Med Sci Monit 11:BR439-48 (2005). PubMed: 16319780