Anti-Glycogen synthase 1/GYS1 (phospho S640) 抗体 (ab2479)

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Key features and details

  • Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S640)
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody
    Glycogen synthase 1/GYS1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S640)

  • 由来種

    Rabbit

  • 特異性

    This phospho specific polyclonal antibody is specific for phosphorylated Ser 640 of human muscle glycogen synthase (GYS1). Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human Glycogen synthase 1/GYS1 aa 631-642 conjugated to keyhole limpet haemocyanin.
    Sequence: AQGYRYPRPASpVP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項


    Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.

     This product was previously labelled as Glycogen synthase 1

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000.
Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.

ターゲット情報

  • 機能

    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

  • パスウェイ

    Glycan biosynthesis; glycogen biosynthesis.

  • 関連疾患

    Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.

  • 配列類似性

    Belongs to the glycosyltransferase 3 family.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Glycogen [starch] synthase antibody
    • Glycogen synthase 1 (muscle) antibody
    • Glycogen synthase 1 antibody
    • GSY antibody
    • GYS antibody
    • Gys1 antibody
    • GYS1_HUMAN antibody
    • muscle antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)

    Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

  • Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)
    Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)
    All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479) at 1/1000 dilution

    Lanes 1 & 5 : mock treated mouse cardiac myocyte lysate at 12 µl
    Lane 2 : insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
    Lane 3 : insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
    Lane 4 : insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µl
    Lane 6 : CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µl
    Lane 7 : CLA treated (20 nM, 45min) mouse cardiac myocyte lysate
    Lane 8 : CLA treated (100 nM, 45min) mouse cardiac myocyte lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution


    Rabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot.  Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'. 

    Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.

    A 4-20% Criterion gel for SDS-PAGE was used.

参考文献

ab2479 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2479 は 4 報の論文で使用されています。

  • Eckle T  et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). PubMed: 22504483
  • Ambacher KK  et al. The JNK- and AKT/GSK3ß- signaling pathways converge to regulate Puma induction and neuronal apoptosis induced by trophic factor deprivation. PLoS One 7:e46885 (2012). PubMed: 23056511
  • Singh V  et al. Somatostatin receptor subtype-2-deficient mice with diet-induced obesity have hyperglycemia, nonfasting hyperglucagonemia, and decreased hepatic glycogen deposition. Endocrinology 148:3887-99 (2007). PubMed: 17525126
  • Alemzadeh R & Tushaus K Diazoxide attenuates insulin secretion and hepatic lipogenesis in zucker diabetic fatty rats. Med Sci Monit 11:BR439-48 (2005). PubMed: 16319780

レビューと Q&A

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Question

Hi to whom it may concerned: 1) May I have a 10ul-20ul sample to try for western blot? I have used pGS antibody. It wasn't specific. 2) What is the reference for pGS antibody images showed in datasheet ( ab2479)?

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Answer

All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). This antibody has been tested for Western blot application, so it should work nicely. There is no published reference available yet regarding this antibody. We will update the on-line datasheet of this product as soon as get more data. Since mouse cardiac myocyte lysate was used to test this antibody, we would suggest using it as positive control. One more comment: If you are using an antibody that is specific to a phosphorylated epitope, do not use milk to block. Kinases from the milk will non-specifically phosphorylate proteins in your samples. Use BSA as a blocking agent. It is also important to block overnight.

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