Anti-Glycogen synthase 1/GYS1 (phospho S640) 抗体 (ab2479)
製品の概要
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製品名
Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody
Glycogen synthase 1/GYS1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S640) -
由来種
Rabbit -
特異性
This phospho specific polyclonal antibody is specific for phosphorylated Ser 640 of human muscle glycogen synthase (GYS1). Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Glycogen synthase 1/GYS1 aa 631-642 conjugated to keyhole limpet haemocyanin.
Sequence: AQGYRYPRPASpVP -
特記事項
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
This product was previously labelled as Glycogen synthase 1
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
一次抗体 備考
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000. Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
ターゲット情報
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機能
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. -
パスウェイ
Glycan biosynthesis; glycogen biosynthesis. -
関連疾患
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work. -
配列類似性
Belongs to the glycosyltransferase 3 family. - Information by UniProt
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参照データベース
- Entrez Gene: 2997 Human
- Entrez Gene: 14936 Mouse
- Entrez Gene: 690987 Rat
- Omim: 138570 Human
- SwissProt: P13807 Human
- SwissProt: Q9Z1E4 Mouse
- SwissProt: A2RRU1 Rat
- Unigene: 386225 Human
see all -
別名
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479) at 1/1000 dilution
Lanes 1 & 5 : mock treated mouse cardiac myocyte lysate at 12 µl
Lane 2 : insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 3 : insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 4 : insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 6 : CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µl
Lane 7 : CLA treated (20 nM, 45min) mouse cardiac myocyte lysate
Lane 8 : CLA treated (100 nM, 45min) mouse cardiac myocyte lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilutionRabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.
A 4-20% Criterion gel for SDS-PAGE was used.
データシートおよび資料
参考文献
This product has been referenced in:
- Eckle T et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). Read more (PubMed: 22504483) »
- Ambacher KK et al. The JNK- and AKT/GSK3ß- signaling pathways converge to regulate Puma induction and neuronal apoptosis induced by trophic factor deprivation. PLoS One 7:e46885 (2012). Read more (PubMed: 23056511) »
