Glycogen Assay Kit II (Colorimetric) (ab169558)
製品の概要
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製品名
Glycogen Assay Kit II (Colorimetric)
Glycogen キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
> 4 µg/ml -
検出範囲
4 µg/ml - 40 µg/ml -
全工程の試験時間
1h 00m -
種交差性
交差種: Other species, Mammals -
製品の概要
Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples.
This glycogen assay is suitable for measuring glycogen levels in samples that contain reducing substances, which may interfere with oxidase-based assays (such as our most popular glycogen assay kit ab65620).
In this glycogen assay protocol, glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless probe to a colored product with strong absorbance at 450 nm. This high-throughput suitable assay kit can detect 4 - 40 µg/ml of glycogen in samples.
Glycogen assay protocol summary:
- add samples and standards to wells
- add hydrolysis enzyme and incubate for 30 min at room temp
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader -
特記事項
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 100 tests Development Enzyme Mix (lyophilized) Green 1 vial Glycogen Development Buffer 1 x 25ml Glycogen Hydrolysis Buffer 1 x 25ml Glycogen Standard (2.0 mg/ml) Yellow 1 x 100µl Hydrolysis Enzyme Mix (lyophilized) Blue 1 vial Probe 1 vial -
研究分野
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関連性
Glycogen is the primary short term energy storage molecule in animals. It is synthesized primarily in the liver and muscle. Glycogen is a highly branched polymer of glucose molecules, connected with an alpha-1,4 linkage, branching via an alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.
画像
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Glycogen measured in cell lysates showing quantity (�g) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL (HeLa) and 6.6e6 cells/mL (HCT116) were used. Samples were diluted 2-6 fold.
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Glycogen measured in tissue lysates showing quantity (�g) per mg of extracted protein.
Protein concentration for samples varied from 25 mg/mL to 50 mg/mL. Samples were diluted 2-54 fold.
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
プロトコール
参考文献
This product has been referenced in:
- Zhang Q et al. Lack of muscle mTOR kinase activity causes early onset myopathy and compromises whole-body homeostasis. J Cachexia Sarcopenia Muscle 10:35-53 (2019). Read more (PubMed: 30461220) »
- Yang Q et al. PRKAA1/AMPKa1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis. Nat Commun 9:4667 (2018). Read more (PubMed: 30405100) »