Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6567] to Glycerol kinase - BSA and Azide free
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-Glycerol kinase antibody [EPR6567] - BSA and Azide free
Glycerol kinase 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6567] to Glycerol kinase - BSA and Azide free
アプリケーション適用あり: Flow Cyt, IP, WBmore details
適用なし: ICC,ICC/IF or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Glycerol kinase aa 450-550. The exact sequence is proprietary.
Ab248122 is the carrier-free version of ab126599. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248122 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 4.80 x 10 -11 M Learn more about KD
Concentration information loading...
精製度Protein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab248122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).|
機能Key enzyme in the regulation of glycerol uptake and metabolism.
組織特異性Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
パスウェイPolyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
関連疾患Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
配列類似性Belongs to the FGGY kinase family.
細胞内局在Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
- Information by UniProt
- ATP glycerol 3 phosphotransferase antibody
- ATP:glycerol 3 phosphotransferase antibody
- ATP:glycerol 3-phosphotransferase antibody
All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
This data was developed using ab126599, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.