Anti-Glycerol kinase 抗体 [EPR6567] - BSA and Azide free (ab248122)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6567] to Glycerol kinase - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Glycerol kinase antibody [EPR6567] - BSA and Azide free
Glycerol kinase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6567] to Glycerol kinase - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Mouse adipose tissue lysate. Fetal liver, HEK-293T, Jurkat and HepG2 whole cell lysate (ab7900). Flow Cyt (intra): Permeabilized HepG2 cells.
-
特記事項
ab248122 is the carrier-free version of ab126599.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 4.80 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6567 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
KO cell lines
-
KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248122の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
|
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Key enzyme in the regulation of glycerol uptake and metabolism. -
組織特異性
Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver. -
パスウェイ
Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. -
関連疾患
Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only. -
配列類似性
Belongs to the FGGY kinase family. -
細胞内局在
Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 2710 Human
- Entrez Gene: 14933 Mouse
- Entrez Gene: 79223 Rat
- Omim: 300474 Human
- SwissProt: P32189 Human
- SwissProt: Q64516 Mouse
- SwissProt: Q63060 Rat
- Unigene: 1466 Human
see all -
別名
- ATP glycerol 3 phosphotransferase antibody
- ATP:glycerol 3 phosphotransferase antibody
- ATP:glycerol 3-phosphotransferase antibody
see all
画像
-
All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : GK knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDaThis data was developed using ab126599, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab126599 observed at 61 kDa. Red - loading control ab8245 observed at 36 kDa.
ab126599 Anti-Glycerol kinase antibody [EPR6567] was shown to specifically react with Glycerol kinase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab267328 (knockout cell lysate ab257966) was used. Wild-type and Glycerol kinase knockout samples were subjected to SDS-PAGE. ab126599 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?This data was developed using ab126599, the same antibody clone in a different buffer formulation.
-
This data was developed using ab126599, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab248122 は論文での使用が確認できていません。