製品の概要

  • 製品名
    Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay Kit (Colorimetric)
    GAPDH キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプ
    Enzyme activity
  • 種交差性
    交差種: Other species, Mammals
  • 製品の概要

    Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay Kit (Colorimetric) (ab204732) provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 100 µU/mL in a variety of samples.

  • 特記事項

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.

  • 試験プラットフォーム
    Microplate

製品の特性

関連製品

画像

  • Typical NADH Standard Curve.

  • GAPDH activity in the Positive Control, 3T3 cells lysate and MDA-MB-468 cells lysate.

  • GAPDH specific activity calculated from 3T3 cell lysate (11.1 µg protein), and MDA-MB-468 cell lysate (2.76 µg protein).

プロトコール

参考文献

This product has been referenced in:
  • Ebner P  et al. Lantibiotic production is a burden for the producing staphylococci. Sci Rep 8:7471 (2018). Read more (PubMed: 29749386) »
  • Tallima H  et al. Protective immune responses against Schistosoma mansoni infection by immunization with functionally active gut-derived cysteine peptidases alone and in combination with glyceraldehyde 3-phosphate dehydrogenase. PLoS Negl Trop Dis 11:e0005443 (2017). Read more (PubMed: 28346516) »
See all 3 Publications for this product

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