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リコンビナントRabMAb

Anti-Glutathione S Transferase mu 抗体 [EPR1936(2)] (ab108524)

Reviews (3) Submit a question References (1)
Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
  • Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
  • Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
  • Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
  • Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1936(2)] to Glutathione S Transferase mu
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Glutathione S Transferase mu antibody [EPR1936(2)]
    Glutathione S Transferase mu 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1936(2)] to Glutathione S Transferase mu

  • 由来種

    Rabbit

  • 特異性

    ab108524 is an Anti-GSTM1 antibody, but it’s immunogen shares 85% homology with GSTM5. Cross-reactivity with GSTM5 has not been confirmed experimentally.

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HepG2, SH-SY5Y and NIH/3T3 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108524の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

  • 組織特異性

    Liver (at protein level).

  • 配列類似性

    Belongs to the GST superfamily. Mu family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Glutathione S alkyltransferase antibody
    • Glutathione S aralkyltransferase antibody
    • Glutathione S aryltransferase antibody
    • Glutathione S transferase M1 antibody
    • Glutathione S transferase mu 1 antibody
    • Glutathione S transferase mu antibody
    • Glutathione S-transferase Mu 1 antibody
    • GST class mu 1 antibody
    • GST class Mu antibody
    • GST class-mu 1 antibody
    • GST HB subunit 4 antibody
    • GST mu antibody
    • GST1 antibody
    • GSTM1 1 antibody
    • Gstm1 antibody
    • GSTM1-1 antibody
    • GSTM1_HUMAN antibody
    • GSTM1a 1a antibody
    • GSTM1a-1a antibody
    • GSTM1b 1b antibody
    • GSTM1b-1b antibody
    • GTH4 antibody
    • GTM1 antibody
    • H B antibody
    • HB subunit 4 antibody
    • MGC26563 antibody
    • MU 1 antibody
    • MU antibody
    • S (hydroxyalkyl)glutathione lyase antibody
    see all

画像

  • Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (purified)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : SH-SY5Y whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking and dilution buffer: 5% NFDM/TBST
  • Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + NIH/3T3 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking and dilution buffer: 5% NFDM/TBST
  • Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + PC-12 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 26 kDa

  • Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
    Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)

参考文献 (1)

ab108524 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108524 は 1 報の論文で使用されています。

  • Kratzer I  et al. Glutathione Conjugation at the Blood-CSF Barrier Efficiently Prevents Exposure of the Developing Brain Fluid Environment to Blood-Borne Reactive Electrophilic Substances. J Neurosci 38:3466-3479 (2018). PubMed: 29507144

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Glutathione S Transferase mu antibody [EPR1936(2)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 18 2016

Western blot abreview for Anti-Glutathione S Transferase mu antibody [EPR1936(2)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 18 2016

Western blot abreview for Anti-Glutathione S Transferase mu antibody [EPR1936(2)]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 04 2014

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