Anti-Glutathione Peroxidase 4 抗体 [1H11] (ab16739)
![Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)](https://www.abcam.co.jp/ps/products/16/ab16739/Images/ab16739-297384-anti-glutathione-peroxidase-4-antibody-1h11-immunoprecipitation.jpg "Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)")
Key features and details
- Mouse monoclonal [1H11] to Glutathione Peroxidase 4
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Glutathione Peroxidase 4 antibody [1H11]
Glutathione Peroxidase 4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1H11] to Glutathione Peroxidase 4 -
由来種
Mouse -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein purified from E.coli (Human).
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ポジティブ･コントロール
- HL60 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
1H11 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16739の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use a concentration of 1 - 2 µg/ml.
|
| 特記事項 |
|---|
|
IP
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. -
組織特異性
Present primarily in testis. -
配列類似性
Belongs to the glutathione peroxidase family. -
細胞内局在
Mitochondrion. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2879 Human
- Omim: 138322 Human
- SwissProt: P36969 Human
- Unigene: 433951 Human
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別名
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
see all
画像
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Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)
Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175
All lanes :
Lane 1 : 239T Cell Lysate
Lane 2 : Precipitated from 239T Cell Lysate (using 2 ug ab16739) at 200 µg
Lane 3 : Precipitated from 239T Cell Lysate (using 5 ug ab16739) at 200 µg
Lane 4 : Precipitated from PBS (using 5 ug ab16739)
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/5000 dilution -
![Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)](https://www.abcam.co.jp/ps/products/16/ab16739/Images/ab16739-25589-anti-glutathione-peroxidase-4-antibody-1h11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 4 antibody [1H11] (ab16739)Ab16739 at a concentration of 1µg/ml lysate immunoprecipitating approx. 22kDa Glutathione peroxidase 4 from HL60 cell lysate (lane 2). Ab16800 was used to stain the Western blot. Lane 1) contains non-immunoprecipitated HL60 cell lysate.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab16739 は 2 報の論文で使用されています。
- Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
- Yang L et al. Broad Spectrum Deubiquitinase Inhibition Induces Both Apoptosis and Ferroptosis in Cancer Cells. Front Oncol 10:949 (2020). PubMed: 32596160
