Anti-Glutathione Peroxidase 3/GPx-3 抗体 [EPR22815-112] (ab256470)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22815-112] to Glutathione Peroxidase 3/GPx-3
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112]
Glutathione Peroxidase 3/GPx-3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22815-112] to Glutathione Peroxidase 3/GPx-3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IHC-Frmore details
適用なし: IP -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse and rat kidney tissue lysate. IHC-P: Mouse and rat kidney and lung tissue. IHC-Fr: Mouse and rat kidney tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22815-112 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab256470の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 22, 24 kDa (predicted molecular weight: 25 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/100.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
特記事項 |
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WB
1/1000. Detects a band of approximately 22, 24 kDa (predicted molecular weight: 25 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ターゲット情報
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機能
Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. -
組織特異性
Secreted in plasma. -
配列類似性
Belongs to the glutathione peroxidase family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 14778 Mouse
- Entrez Gene: 64317 Rat
- SwissProt: P46412 Mouse
- SwissProt: P23764 Rat
- Unigene: 200916 Mouse
- Unigene: 108074 Rat
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別名
- Extracellular glutathione peroxidase antibody
- Glutathione peroxidase 3 (plasma) antibody
- Glutathione peroxidase 3 antibody
see all
画像
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All lanes : Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 22-24 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsThe expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20015939).
The 22-KDa band represents another Gpx3 isoform.
Negative control: Mouse liver (PMID: 15294905).
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Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] (ab256470) at 1/1000 dilution (Purified) + Rat kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking and dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20015939).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/100 (4.80 μg/ml) dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining on rat kidney (PMID: 21493731) is observed.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
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Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/100 (4.80 μg/ml) dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining on mouse kidney (PMID: 21493731) is observed.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
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Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat lung (PMID: 22355371) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat kidney (PMID: 21493731, 27435875) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung (PMID: 22355371) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
-
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID: 21493731, 27435875) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (5)
ab256470 は 5 報の論文で使用されています。
- Liu L et al. Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration. Biomol Biomed 23:101-113 (2023). PubMed: 35997993
- Tang LX et al. Intercellular mitochondrial transfer as a means of revitalizing injured glomerular endothelial cells. World J Stem Cells 14:729-743 (2022). PubMed: 36188114
- Refaie MMM et al. Dapagliflozin Guards Against Cadmium-Induced Cardiotoxicity via Modulation of IL6/STAT3 and TLR2/TNFα Signaling Pathways. Cardiovasc Toxicol 22:916-928 (2022). PubMed: 36242756
- Zhang F et al. PARK7 promotes repair in early steroid-induced osteonecrosis of the femoral head by enhancing resistance to stress-induced apoptosis in bone marrow mesenchymal stem cells via regulation of the Nrf2 signaling pathway. Cell Death Dis 12:940 (2021). PubMed: 34645791
- Li Z et al. Effect of fosinopril on the renal cortex protein expression profile of Otsuka Long-Evans Tokushima Fatty rats. Exp Ther Med 19:172-182 (2020). PubMed: 31853288