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遺伝子ノックアウト細胞株 検証済

Anti-Glutathione Peroxidase 1 抗体 (ab22604)

  • Datasheet
  • SDS
Reviews (23)Q&A (17)References (114)

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Immunocytochemistry - Anti-Glutathione Peroxidase 1 antibody (ab22604)
  • Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
  • Immunocytochemistry - Anti-Glutathione Peroxidase 1 antibody (ab22604)
  • Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
  • Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)

Key features and details

  • Rabbit polyclonal to Glutathione Peroxidase 1
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Glutathione Peroxidase 1 antibody
    Glutathione Peroxidase 1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Glutathione Peroxidase 1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide within Human Glutathione Peroxidase 1 aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P07203

  • ポジティブ・コントロール

    • ICC: HepG2 cells. WB: Wild-type HAP1 cell lysate. Human, mouse and rat liver tissue lysate.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
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    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Glutathione Peroxidase 1 protein (ab113603)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab22604の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use a concentration of 5 µg/ml.
WB (11)
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
特記事項
ICC
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

ターゲット情報

  • 機能

    Protects the hemoglobin in erythrocytes from oxidative breakdown.
  • 配列類似性

    Belongs to the glutathione peroxidase family.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession P07203 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 281209 Cow
    • Entrez Gene: 2876 Human
    • Entrez Gene: 14775 Mouse
    • Entrez Gene: 24404 Rat
    • Omim: 138320 Human
    • SwissProt: P00435 Cow
    • SwissProt: P07203 Human
    • SwissProt: P11352 Mouse
    • SwissProt: P04041 Rat
    • Unigene: 76686 Human
    • Unigene: 1090 Mouse
    • Unigene: 11323 Rat
    see all
  • 別名

    • AL033363 antibody
    • Cellular glutathione peroxidase antibody
    • Glutathione peroxidase 1 antibody
    • Glutathione peroxidase antibody
    • GPx 1 antibody
    • GPx-1 antibody
    • GPX1 antibody
    • GPX1_HUMAN antibody
    • GPXD antibody
    • GSHPx-1 antibody
    • GSHPX1 antibody
    • MGC14399 antibody
    • MGC88245 antibody
    see all

画像

  • Immunocytochemistry - Anti-Glutathione Peroxidase 1 antibody (ab22604)
    Immunocytochemistry - Anti-Glutathione Peroxidase 1 antibody (ab22604)

    ab22604 staining Glutathione Peroxidase 1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab22604 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
    Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
    Lane 3: THP1 cell lysate (20 µg)
    Lane 4: HL60 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab22604 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab22604 was shown to recognize Glutathione Peroxidase 1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab22604 at a concentration of 1µg/ml and ab8245 (loading control to GAPDH) diluted at 1/2000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-Glutathione Peroxidase 1 antibody (ab22604)
    Immunocytochemistry - Anti-Glutathione Peroxidase 1 antibody (ab22604)This image is courtesy of an Abreview submitted by Carl Hobbs

    ab22604 staining mouse Cor-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with TBS/BSA/azide/0.1%Tween 20 and blocked with 1% BSA for 10 minutes at RT. Samples were incubated with primary antibody (1/300) for 2 hours. An diluted (1/1000) Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
    Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
    All lanes : Anti-Glutathione Peroxidase 1 antibody (ab22604) at 1 µg/ml (Blocked with 3% Milk)

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 190 kDa, 55 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab22604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
    Western blot - Anti-Glutathione Peroxidase 1 antibody (ab22604)
    All lanes : Anti-Glutathione Peroxidase 1 antibody (ab22604) at 1 µg/ml

    Lane 1 : Human liver normal tissue lysate
    Lane 2 : Liver (Mouse) Tissue Lysate (ab7935)
    Lane 3 : Human liver normal tissue lysate with Human Glutathione Peroxidase 1 peptide (ab25301) at 1 µg/ml
    Lane 4 : Liver (Mouse) Tissue Lysate (ab7935) with Human Glutathione Peroxidase 1 peptide (ab25301) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22,24 kDa why is the actual band size different from the predicted?



    ab22604 detects a band of approximately 22 kDa in human liver lysate. The band detected in the mouse liver lysate runs slightly higher (~ 24kDa). We believe that these bands correspond to Glutathione Peroxidase 1 as they are both blocked by the addition of the immunizing peptide (ab25301).

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (114)

ab22604 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab22604 は 114 報の論文で使用されています。

  • Thangaraj A  et al. HIV TAT-mediated microglial senescence: Role of SIRT3-dependent mitochondrial oxidative stress. Redox Biol 40:101843 (2021). PubMed: 33385630
  • Fang X  et al. N-acetylcysteine inhibits atherosclerosis by correcting glutathione-dependent methylglyoxal elimination and dicarbonyl/oxidative stress in the aorta of diabetic mice. Mol Med Rep 23:N/A (2021). PubMed: 33495825
  • Reis-Mendes A  et al. Inflammation as a Possible Trigger for Mitoxantrone-Induced Cardiotoxicity: An In Vivo Study in Adult and Infant Mice. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34073506
  • Almada-Érix CN  et al. Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity. Front Microbiol 12:623951 (2021). PubMed: 34135869
  • Montalvo RN  et al. Doxorubicin-induced oxidative stress differentially regulates proteolytic signaling in cardiac and skeletal muscle. Am J Physiol Regul Integr Comp Physiol 318:R227-R233 (2020). PubMed: 31774307
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 40 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutathione Peroxidase 1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid 4%
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Miss. Jin Young Park

Verified customer

投稿 Aug 28 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutathione Peroxidase 1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid 4%
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jul 04 2017

Western blot abreview for Anti-Glutathione Peroxidase 1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Julie Kim

Verified customer

投稿 May 12 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutathione Peroxidase 1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate acid solution
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Miss. Jin Young Park

Verified customer

投稿 Feb 08 2017

Flow Cytometry abreview for Anti-Glutathione Peroxidase 1 antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (ovarian cancer (OVCAr-3))
Permeabilization
Yes - Ethanol 70%, 30 min at -20
Specification
ovarian cancer (OVCAr-3)
Read More

Abcam user community

Verified customer

投稿 Nov 25 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glutathione Peroxidase 1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Antigen retrieval step
None
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 15 2016

Western blot abreview for Anti-Glutathione Peroxidase 1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 23 2016

Western blot abreview for Anti-Glutathione Peroxidase 1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 18 2016

Question

DESCRIPTION OF THE PROBLEM No signal or weak signal
SAMPLE HCC cell-whole cell lysates, Human liver tissue- whole cell lysates
PRIMARY ANTIBODY Concentration or dilution 1:1000 Diluent buffer: 5% skim milk+0.1%PBST+ Ab+0.02% sodium azide Incubation time: 1hr or Overnight Incubation temperature: RT for 1hr and 4℃ for O/NBuffer: 0.1% TBST Number of washes: 5min/time * 4times
DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED Positive control beta-actin and SOD2, SOD1, catalase Negative control No
ANTIBODY STORAGE CONDITIONS Aliquot and store at -80℃
SAMPLE PREPARATION Lysis buffer : RIPA buffer Phosphatase inhibitors: Protease Inhibitor Cocktail 25955-11 (Nacalai USA,Inc.) Reducing agent: BME Boiling for ≥5 min? yes
AMOUNT OF PROTEIN LOADED 20ug/lane
ELECTROPHORESIS/GEL CONDITIONS 12% TRANSFER AND BLOCKING CONDITIONS Type of membrane PVDF membrane Protein transfer verified Bio-Rad
SEMI DRY TRANSFER 20V 1hr 4℃ Blocking agent and concentration 5% Skim milk in 0.1% PBST Blocking time 30min Blocking temperature RT SECONDARY ANTIBODY Secondary antibody: Goat polyclonal to Rabbit IgG Species: Goat Isotype: IgG Reacts against: anti-Rabbit IgG Concentration or dilution 1:10000 Diluent buffer 0.1% PBST Incubation time: 1hr Incubation temperature: RT Fluorochrome or enzyme conjugate: HRP Buffer: 0.1% TBST Number of washes: 5min/time * 4times
HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes
DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No
WHAT STEPS HAVE YOU ALTERED? Increased the dilution ratio of primary Ab to 1:500 and the transfer time to 1hr 30min, however, the results were all the same.
ADDITIONAL NOTES -

Read More

Abcam community

Verified customer

Asked on Apr 09 2012

Answer

Thank you for submitting your completed questionnaire.

It does appear that there is a problem with the vial of ab22604 you received. As you have purchased this antibody within the last 6 months I would be happy to offer a replacement or credit note. Please let me know how you wisht to proceed.

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Abcam Scientific Support

Answered on Apr 09 2012

Question

please contact with us. I want to purchase the above chemicals as soon as possible.

Read More

Abcam community

Verified customer

Asked on Jan 04 2012

Answer

Thank you for contacting us. Please contact our Indian distributor to buy these products. You can’t purchase products directly fromAbcam. The contact addressof Indian distributor is Allied Scientific Products 39/19B Gopal Nagar Road Kolkata-700027 India Website http://www.alliedscientific.net/ Freephone 1800 345 7777 Fax +91 33 24566683 / 24790626 Tel +91 33 24566684/24793109/9039 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

Answered on Jan 04 2012

1-10 of 40 Abreviews or Q&A

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