Anti-Glutamine Synthetase 抗体 [EPR13022(B)] - BSA and Azide free (ab240193)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase - BSA and Azide free
- Suitable for: mIHC, WB, IHC-P, IHC-Fr
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free
Glutamine Synthetase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: mIHC, WB, IHC-P, IHC-Frmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal liver lysate, mouse and rat spleen lysate Jurkat, HAP1 and HeLa whole cell lysate (ab150035); IHC-P: Human glioma and liver tissues, mouse liver tissue; IHC-Fr: Rat and Mouse cerebrum. mIHC: Human retina tissue.
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特記事項
ab240193 is the carrier-free version of ab176562.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13022(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562)
- HRP Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab199198)
- Alexa Fluor® 647 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab300751)
- Alexa Fluor® 555 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab302572)
- Alexa Fluor® 488 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab302584)
- Alexa Fluor® 568 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab302807)
- Alexa Fluor® 594 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab305118)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab240193の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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mIHC |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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mIHC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
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IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
関連疾患
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
配列類似性
Belongs to the glutamine synthetase family. -
発生段階
Expressed during early fetal stages. -
細胞内局在
Cytoplasm. Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 2752 Human
- Entrez Gene: 14645 Mouse
- Entrez Gene: 24957 Rat
- Omim: 138290 Human
- SwissProt: P15104 Human
- SwissProt: P15105 Mouse
- SwissProt: P09606 Rat
- Unigene: 518525 Human
see all -
別名
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
画像
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This data was developed using ab176562, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human retina tissue labeling PAX6, Glutamine Synthetase and CRX with ab109233 at 1/10000 dilution, ab240193 at 1/20000 dilution and ab248897 at 1/1000 dilution followed by a ready to use Opal Polymer HRP Ms + Rb secondary antibody. Nuclear counter stain used was DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Panel A: merged staining of anti-CRX (gray; Opal™690), anti-Glutamine Synthetase (green; Opal™520) and anti-PAX6 (red; Opal™570) on human retina.
Panel B: anti-PAX6 stained on retinal progenitor cells.
Panel C: anti-Glutamine Synthetase stained on Müller glia.
Panel D: anti-CRX stained on subset cells of outer nuclear layer and inner nuclear layer.The section was incubated in three rounds of staining: in the order of ab248897, ab240193, and ab109233 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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All lanes : Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) at 1/2000 dilution
Lane 1 : Human fetal liver lysates at 20 µg
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 3 : Mouse spleen lysates at 20 µg
Lane 4 : Rat spleen lysates at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaThis data was developed using ab176562, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDaThis data was developed using ab176562, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GLUL knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 42 kDaThis data was developed using ab176562, the same antibody clone in a different buffer formulation:
Lanes 1 - 2: Merged signal (red and green). Green - ab176562 observed at 42 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab176562 was shown to recognize Glutamine Synthetase in wild-type HAP1 cells as signal was lost at the expected MW in GLUL knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLUL knockout samples were subjected to SDS-PAGE. Ab176562 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilised mouse cerebrum tissue staining glutamine synthetase with ab176562 at 1/250 dilution, followed by alexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). DAPI was used as a nuclear counterstain.
Positive staining on mouse cerebrum (PMID: 23895693).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human glioma tissue labeling Glutamine Synthetase with unpurified ab176562 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Glutamine Synthetase with purified ab176562 at 1:500 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilised rat cerebrum tissue staining glutamine synthetase with ab176562 at 1/250 dilution, followed by alexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). DAPI was used as a nuclear counterstain.
Positive staining on rat cerebrum (PMID: 23895693).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human liver tissue labeling Glutamine Synthetase with unpurified ab176562 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab176562 staining Glutamine Synthetasein Mouse Liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with PB ab64226 for 10 minutes at room temperature and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176562).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab240193 は論文での使用が確認できていません。