Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (P5 cerebellum)
Specification
P5 cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit IgGs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
This Ab can be diluted further.
Submitted image shows clear positivity of Bergmann glia cell bodies (indicated by green arrowheads) and their processes, the major process extending from the cell body in/about the Purkinje cell layer (red arrowhead) through the developing Molecular layer (ML - indicated by a blue double-headed arrow) to the surface of the folium, where the endfeet will terminate on the inner meningial layer (pia mater).
One can easily see many nuclei (blue dots) in the outer Molecular layer: these young neurones will migrate to the Granule Cell layer (red:GC), apparently using the Bergmann glia processes for guidance.
In the white matter (WM: red) and the developing Granule cell layer many positive cells are also seen (black arrowheads) presumably they are Type 1 Astrocytes.
Submitted image shows clear positivity of Bergmann glia cell bodies (indicated by green arrowheads) and their processes, the major process extending from the cell body in/about the Purkinje cell layer (red arrowhead) through the developing Molecular layer (ML - indicated by a blue double-headed arrow) to the surface of the folium, where the endfeet will terminate on the inner meningial layer (pia mater).
One can easily see many nuclei (blue dots) in the outer Molecular layer: these young neurones will migrate to the Granule Cell layer (red:GC), apparently using the Bergmann glia processes for guidance.
In the white matter (WM: red) and the developing Granule cell layer many positive cells are also seen (black arrowheads) presumably they are Type 1 Astrocytes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Jul 19 2010