製品の概要

  • 製品名
    Anti-Glutamine Synthetase antibody
    Glutamine Synthetase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutamine Synthetase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-FoFr, IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, Common marmoset
    交差が予測される動物種: Hamster, Cow, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Mouse Glutamine Synthetase aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: P15105
    (Peptide available as ab73592)

  • ポジティブ・コントロール
    • WB: Mouse kidney, mouse liver, rat liver, human brain, mouse brain, rat lung and mouse pancreas (data not shown) tissue lysates. ICC/IF: MEF1 cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/3000.
IHC-P 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Mouse Glutamine Synthetase peptide (ab73592).

ターゲット情報

  • 機能
    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • 関連疾患
    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • 配列類似性
    Belongs to the glutamine synthetase family.
  • 発生段階
    Expressed during early fetal stages.
  • 細胞内局在
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

画像

  • Immunohistochemistical detection of Glutamine Synthetase on Formaldehyde-fixed paraffin-embedded rat brain sections using Glutamine Synthetase antibody (ab73593).  Antigen retrieval step: Heat mediated - Buffer/Enzyme Used: Citric acid pH6 Permeabilization. Blocking: 1% BSA for 10 mins at room temperature. Primary Antibody Dilution 1/1000. Incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG Conjugated to biotin (1/200). In the submitted image of rat cerebellum, two very good examples are observed of the bulbous endfeet of the primary Bergmann glia primary processes connecting with the inner Pial membrane.

  • All lanes : Anti-Glutamine Synthetase antibody (ab73593) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Brain (Mouse) Tissue Lysate
    Lane 6 : Lung (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 30 seconds
  • ab73593 staining Glutamine Synthetase in MEF1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab73593 at 5ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

  • IHC-FoFr image of Glutamine Synthetase (ab73593) staining on Rat hippocampus sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

  • Anti-Glutamine Synthetase antibody (ab73593) at 1 µg/ml + Recombinant Human Glutamine Synthetase protein (ab98145) at 0.01 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa


    Exposure time: 2 minutes

参考文献

This product has been referenced in:
  • Rocchio F  et al. Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer's disease mouse model. Cell Death Dis 10:24 (2019). Read more (PubMed: 30631041) »
  • Zou T  et al. Organoid-derived C-Kit+/SSEA4- human retinal progenitor cells promote a protective retinal microenvironment during transplantation in rodents. Nat Commun 10:1205 (2019). Read more (PubMed: 30872578) »
See all 28 Publications for this product

レビューと Q&A

1-10 of 13 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Sep 10 2018

Application
Western blot
Sample
Rat Cell lysate - whole cell (neuronal cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
neuronal cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Mar 27 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (B lymphoblast)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
B lymphoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jan 19 2017

Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Muller Cell)
Specification
Retina, Muller Cell
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Ryan Macdonald

Verified customer

投稿 May 28 2013

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Jan 28 2013

Answer

Thank you for your inquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 6 months from the date of purchase when stored correctly. For ab73593, we recommend storing at either -20C or -80C and at these temperatures the antibody could be viable up to 5 - 10 years.

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

Answer

Thank you for contacting us in regards to anti-Glutamine Synthetase antibody (ab73593).

I now have confirmation form the lab of how the Western blot presented on the datasheet for this antibody was performed. We used 10 ug of the different tissue lysates with 1 ug/ml of primary antibody. The membrane wasblocked for 1 hour at room temperature with 5% BSA in 0.1% TBST, with gentle rocking.Theprimary antibody wasdiluted with 5% BSA in 0.1% TBST and incubated overnight at 4°C with gentle rocking.

We observedvery strong bands after 30 seconds ofexposure (ECL) so it’s possible the amount of antibody could be reduced further. If you are using this antibody in Western blottingwith hamster samples, we would be very interested in how you get on. If you would like,you can submit anAbreview whichwill allow other customer tofind out how you foundusing the antibody. This would only take 5-10 minutes andas a reward you would getAbpointswhich can be redeemed against future purchases, or as Amazon vouchers. More information on this schemecan be found from the following link:

https://www.abcam.com/abreviews

I hope this information has been of help.If you have any problems in using thisantibody in westernblotting pleasedo let us know, or if you require any further information please do not hesitate to contact us again.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain, spinal cord)
Specification
Brain, spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Nov 14 2011

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain - Hippocampus)
Specification
Brain - Hippocampus
Fixative
Paraformaldehyde

Dr. Sophie Pezet

Verified customer

投稿 Sep 08 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (P5 cerebellum)
Specification
P5 cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Mr. Carl Hobbs

Verified customer

投稿 Jul 19 2010

1-10 of 13 Abreviews or Q&A

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