Anti-Glutamine Synthetase 抗体 (ab16802)

Thank you for contacting us.
I am sorry that the antibody is not working as expected.
If you please could send me an image of your staining, that would be of great help.
I'd be happy to further discuss options of resolving this case.
I look forward to hear back from you.

Thank you for contacting us.

We carry 13 antibodies to glutamine synthetase. None of these have so far been tested in yeast.

I have 2 questions for you:

1) What application/technique are you planning to use the antibody in?
2) Could you please provide the yeast GS sequence?

It would be worthwhile to check sequence homology with the immunogen of these antibodies as crossreactivity might be likely. I can check this for you, if you could kindly send me the yeast sequence.

If the homology is 85% or higher, you would be eligible to use our testing discount program. Here is some more information:

For UNTESTED species and/or applications, we have established a testing discount program. A brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I look forward to hear back from you and assist your further.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab49873.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Thank you for your reply.
I think you would not really see an overlap with the nucleus as GS is more located in the cytoplasm and mitochondria, but the staining should be more intense in general.
I'd be happy to replace, credit or refund the antibody. Please let me know which option would be best.
We have the following antibodies that have been tested in IHC-Fr as well as rat: ab64613, ab73593, and ab49873.
I look forward to hear back from you.

Thank you for your email and the image.
The staining does look like non-specific staining. I have a couple questions in this regard:
1) What antibody dilution was used for this image?
2) Where would the nuclei be located in relation to the "red circles"?
3) And where would the GFP be located in relation to these circles?
4) Also, do you have any other image with brighter red staining? Or is it always this dim?
If this image is representative of the results you get, it would indicate a problem with the staining and potentially the primary antibody.
I look forward to hear back from you and assist you further.

Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six month from the date of purchase when stored correctly. For this antibody when stored correctly I would in general expect a shelf life of approximately 5 years. But this can also be longer and has to be experimentally tested in any case. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.