Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S381) - BSA and Azide free
- Suitable for: Dot blot, WB, IP
- Reacts with: Mouse, Rat, Human
製品名Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free
Glutamate Receptor 1 (AMPA subtype) 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S381) - BSA and Azide free
This antibody only detects Glutamate Receptor 1 phosphorylated at Serine 831.
アプリケーション適用あり: Dot blot, WB, IPmore details
適用なし: Flow Cyt,ICC or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Glutamate Receptor 1 (AMPA subtype) (phospho S831). The exact sequence is proprietary.
Ab247874 is the carrier-free version of ab109464. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247874 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).|
|IP||Use at an assay dependent concentration.|
機能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
組織特異性Widely expressed in brain.
配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
翻訳後修飾Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
細胞内局在Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
- Information by UniProt
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Rat brain lysates, untreated
Lane 2 : Rat brain lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
This data was developed using ab109464, the same antibody clone in a different buffer formulation.
The lower panel shows Glutamate Receptor 1 detected with an alternative anti-Glutamate Receptor 1 antibody which is not dependent upon antigen phosphorylation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.